Dicaffeoyl component in walnut green seedcase and production technology and anti-inflammatory application of dicaffeoyl component in walnut green seedcase
A technology of caffeoyl and walnut green skin, applied in the directions of anti-inflammatory agent, sugar derivative preparation, non-central analgesic agent, etc., can solve problems such as insufficient and systematic, and achieve low cost of raw materials, simple process, and anti-inflammatory products. active effect
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Embodiment 1
[0022] Preparation of 1,6-di-O-trans-caffeoyl-3-O-galloyl-β-D-glucopyranoside from walnut green peel of common walnut tree
[0023] (1) Take the walnut green peel of ordinary walnut tree (Juglans regia, also known as walnut, Chinese walnut, walnut, etc.) that has been washed, dried in the shade and crushed through a 60-mesh sieve, and the mass ratio is 1:3 and the volume percentage concentration is 75. % Acetone aqueous solution, microwave-assisted extraction 2 times, 1 hour each time, filtered, the filtrate was concentrated under reduced pressure to 3% of the original volume to obtain a crude extract;
[0024] (2) Add water with twice the weight of the crude extract, stir, add n-hexane with 3 times the weight of the crude extract and extract twice, separate the n-hexane layer, and add ethyl acetate with 3 times the weight of the crude extract to the remaining water layer Ester was extracted twice, the ethyl acetate layer and the water layer were separated, and the petroleum ether ...
Embodiment 2
[0032] Preparation of 1,6-Di-O-trans-caffeoyl-3-O-galloyl-β-D-glucopyranoside from the walnut green bark of the Yangbi foamed walnut tree
[0033] (1) Take the juglans sigillata (also known as Sandai foamed walnut, iron walnut, deep grained foamed walnut, tea foamed walnut, etc.) walnut green peels that have been washed, dried in the shade and crushed through a 60-mesh sieve. Add an 80% acetone aqueous solution with a volume percentage of 1:4, extract 4 times at room temperature for 48 hours each time, filter, and concentrate the filtrate under reduced pressure to 5% of the original volume to obtain a crude extract;
[0034] (2) Add water 3 times the mass of the crude extract, stir, add n-hexane 4 times the mass of the crude extract and extract 3 times, separate the n-hexane layer, and add ethyl acetate 4 times the mass of the crude extract to the remaining water layer Ester extraction was performed 3 times, the ethyl acetate layer and the water layer were separated, and petroleum ...
Embodiment 3
[0037] Evaluation of anti-inflammatory activity of 1,6-di-O-transcaffeoyl-3-O-galloyl-β-D-glucopyranoside
[0038] 1. Sample to be evaluated: The 1,6-di-O-trans-caffeoyl-3-O-galloyl-β-D-glucopyranoside prepared above has a purity of 99.8% determined by HPLC.
[0039] 2. Experimental method: To evaluate the anti-inflammatory activity of the sample to be evaluated, the specific operation strictly follows the steps specified in the literature (Hu W.C, et al, Food & Function, 2016, 7: 1002~1013):
[0040] 3. Test results:
[0041] (1) Evaluate the effect of 1,6-di-O-trans-caffeoyl-3-O-galloyl-β-D-glucopyranoside on the growth of RAW264.7 macrophages by MTT method for 24 hours Impact (toxic effect), the test results are shown in Table 2.
[0042] Table 2. Toxic effects of 1,6-di-O-trans-caffeoyl-3-O-galloyl-β-D-glucopyranoside on RAW267.4 cells
[0043]
[0044] (2) LPS with a concentration of 1ug / mL and 1,6-di-O-trans-caffeoyl-3-O-galloyl-β-D-glucopyranoside were evaluated by Griess method ...
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