Production of lactic acid bacteria fermented liquid high in γ-aminobutyric acid and its application in cosmetics

A technology of aminobutyric acid and fermentation liquid, applied in cosmetics, cosmetic preparations, fermentation and other directions, can solve the problems of low content of functional product γ-aminobutyric acid, limited application, limited types and functions of metabolites, etc., so as to improve the skin. Effects of dullness, improvement of skin whiteness, elasticity and moisture

Active Publication Date: 2020-06-30
哈尔滨美华生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the types and functions of the metabolites after the fermentation of the three fermentation bacteria are limited, and the content of the functional product γ-aminobutyric acid is low, which limits its application in cosmetics

Method used

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  • Production of lactic acid bacteria fermented liquid high in γ-aminobutyric acid and its application in cosmetics
  • Production of lactic acid bacteria fermented liquid high in γ-aminobutyric acid and its application in cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] A lactic acid bacterium produces a fermented liquid high in gamma-aminobutyric acid, the preparation method of which is as follows:

[0053] (1) Germinate brown rice and barley until the leaf bud length is 1 / 3 of the seed, heat to 40°C for 5 minutes, stop germination, and mix with mulberry leaves. The mass ratio of brown rice, barley and mulberry leaves is 1:2:1.5, Pulverize to get mixed powder;

[0054] (2) Soak the mixed powder in water according to the mass volume ratio of 0.25:1g / mL, heat at 55°C for 0.5h, then raise the temperature to 60°C, keep it warm for 2h, and filter while it is hot to obtain the filtrate;

[0055] (3) Add glutamic acid with a mass fraction of 0.5% to the filtrate, heat to 105°C to sterilize for 10 minutes, insert Lactobacillus plantarum, inoculate 1.0%, ferment at 35°C for 6 hours, filter, and the filtrate is produced by lactic acid bacteria Fermentation broth high in γ-aminobutyric acid.

[0056] A cosmetic composition, consisting of the f...

Embodiment 2

[0059] A lactic acid bacterium produces a fermented liquid high in gamma-aminobutyric acid, the preparation method of which is as follows:

[0060] (1) Germinate brown rice and barley until the leaf bud length is 2 / 3 of the seed, heat to 50°C for 15 minutes, stop germination, mix with mulberry leaves, the mass ratio of brown rice, barley and mulberry leaves is 2:1:0.5, Pulverize to get mixed powder;

[0061] (2) Soak the mixed powder in water according to the mass volume ratio of 1.1:1g / mL, heat at 57°C for 1h, then raise the temperature to 65°C, keep it warm for 5h, and filter while it is hot to obtain the filtrate;

[0062] (3) Add sodium glutamate with a mass fraction of 2.5% to the filtrate, heat to 121°C for sterilization for 30 minutes, insert Lactobacillus acidophilus with an inoculum size of 10.0%, ferment at 40°C for 24 hours, filter, and the filtrate is Lactic acid bacteria produce a fermented liquid high in GABA.

[0063] A cosmetic composition, which is composed ...

Embodiment 3

[0066] A lactic acid bacterium produces a fermented liquid high in gamma-aminobutyric acid, the preparation method of which is as follows:

[0067] (1) Germinate brown rice and barley until the leaf bud length is 1 / 2 of the seed, heat to 45°C for 10 minutes, stop germination, mix with mulberry leaves, the mass ratio of brown rice, barley and mulberry leaves is 2:2:1, Pulverize to get mixed powder;

[0068] (2) Soak the mixed powder in water according to the mass volume ratio of 0.7:1g / mL, heat at 56°C for 0.75h, then raise the temperature to 62.5°C, keep it warm for 4h, and filter while it is hot to obtain the filtrate;

[0069] (3) Add glutamic acid with a mass fraction of 1.5% to the filtrate, heat to 113°C to sterilize for 20 minutes, insert Lactobacillus casei, inoculate 5.5%, ferment at 38°C for 15 hours, filter, and the filtrate is produced by lactic acid bacteria Fermentation broth high in γ-aminobutyric acid.

[0070] A cosmetic composition, consisting of the followi...

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Abstract

The invention relates to the field of fermentation technologies and cosmetics, in particular to a preparation method for producing fermentation liquor with a high [gamma]-aminobutyric acid content byemploying lactic acid bacteria, and an application of fermentation liquor in cosmetics. The preparation method comprises the following steps of: (1) enabling brown rice and barley to sprout, mixing the sprouted brown rice and barley with folium mori, and smashing the mixture to obtain a mixed powder; (2) adding water into the mixed powder for dipping, and conducting filtering to obtain a filtrate;and (3) adding glutamic acids or / and glutamate into the filtrate, inoculating fermentative bacteria, and conducting filtering to obtain the fermentation liquor. The fermentation liquor has the advantages of high [gamma]-aminobutyric acid content, good use effect and no side effects when the fermentation liquor is applied to cosmetics.

Description

technical field [0001] The invention relates to the fields of fermentation technology and cosmetics, in particular to the production of fermented liquid containing high gamma-aminobutyric acid by lactic acid bacteria and its application in cosmetics. Background technique [0002] γ-aminobutyric acid (GABA), English name: γ-aminobutyric acid, chemical name: 4-aminobutyric acid, aliases: aminobutyric acid, pipecolic acid, widely distributed in animals and plants. Seeds, rhizomes and tissue fluids of plants such as beans, ginseng, and Chinese herbal medicines all contain GABA. In animals, GABA exists almost only in nerve tissue, and the content in brain tissue is about 0.1-0.6mg / gram tissue. GABA is an important neurotransmitter with a variety of physiologically active functions. It has physiological effects on human epidermal cells, such as physiological repair, cell activation, skin rejuvenation and beauty. GABA is also closely related to the quality of sleep and is known a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/00A61K8/44A61Q19/00C12R1/25C12R1/23C12R1/245C12R1/225
CPCA61K8/44A61K2800/85A61Q19/00C12P13/005
Inventor 梁金钟肖雯娟林枫翔
Owner 哈尔滨美华生物技术股份有限公司
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