Lactobacillus delbrueckii and preparation method and application of direct-to-vat lactobacillus delbrueckii fermenting agent

A technology of Lactobacillus delbrueckii and starter, applied in the field of Lactobacillus delbrueckii, can solve the problems of hidden dangers of health and safety, high monopoly price, etc., and achieve the effects of reducing inoculation, promoting mass growth, and shortening fermentation cycle

Active Publication Date: 2019-12-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When more than 90% of the core strains come from Western human bodies and more than 90% of the core technology is controlled by foreign companies, there are potential health and safety hazards and high monopoly prices

Method used

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  • Lactobacillus delbrueckii and preparation method and application of direct-to-vat lactobacillus delbrueckii fermenting agent
  • Lactobacillus delbrueckii and preparation method and application of direct-to-vat lactobacillus delbrueckii fermenting agent
  • Lactobacillus delbrueckii and preparation method and application of direct-to-vat lactobacillus delbrueckii fermenting agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 A strain of Lactobacillus delbrueckii DMLD-H1 derived from traditional fermented dairy products in China

[0040] This example is a strain of Lactobacillus delbrueckii DMLD-H1 derived from traditional fermented dairy products in China, which is preserved in Guangdong Microbial Culture Collection Center (GDMCC for short) with the preservation number GDMCC 60645. The biological characteristics of the Lactobacillus delbrueckii (Lactobacillus delbrueckii) strain DMLD-H1 are: streaked and separated on the MRS medium plate, cultivated at 37°C for 48 hours, the colonies are round, raised, with neat edges, milky white colonies, and the surface is wet smooth. Gram-stained microscopic examination shows that it is a Gram-positive bacterium with a long rod-shaped body, no flagella, and no spores. Physiological and biochemical test results showed that the strain KOH test was negative, catalase test was negative, facultative anaerobic, able to hydrolyze starch, not liquefy...

Embodiment 2

[0043] Example 2: Safety Evaluation Study of Lactobacillus delbrueckii DMLD-H1

[0044] 2.1 Indigo matrix test

[0045] The activated Lactobacillus delbrueckii DMLD-H1 strain and the quality control strain Staphylococcus aureus were added to the indigo matrix detection medium with an inoculum size of 3%, and incubated at a constant temperature of 37°C for 72h. Then add a small amount of ether to the culture medium, shake and mix well. When the ether layer began to appear on the surface of the culture medium, add 8 drops of Kovacas reagent, observe and take pictures. If the surface of the culture medium is red, the indigo matrix test is positive. The result picture is as follows figure 1 As shown, the contact surface of the quality control strain Staphylococcus aureus showed a red circle, indicating that the result was positive; while the result of Lactobacillus delbrueckii DMLD-H1 was negative, indicating that Lactobacillus delbrueckii DMLD-H1 was safe. Does not break down...

Embodiment 3

[0050] Example 3: Research on high-density culture of Lactobacillus delbrueckii DMLD-H1

[0051] The high-density medium and the production medium are obtained through the following single factor optimization, Plackett-Burmen test and Box-Behnken optimization test, specifically as follows:

[0052] 3.1 Single factor optimization of high-density culture

[0053] 3.1.1 Carbon source optimization

[0054] (1) Glucose optimization

[0055] Depend on Figure 5 It can be seen that when the amount of glucose is increased on the basis of the basic MRS medium, the growth of Lactobacillus delbrueckii DMLD-H1 can be promoted to a certain extent. When adding 2.5 times, that is, 50.0g / L glucose, the number of viable bacteria in the bacterial solution was the highest, which was 6.30×10 8 cfu / mL was 3.06 times that of the blank control. However, when the addition amount was greater than 2.5 times, the number of viable bacteria in the bacterial solution decreased, indicating that too muc...

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Abstract

The invention discloses lactobacillus delbrueckii and a preparation method and application of a direct-to-vat lactobacillus delbrueckii fermenting agent. The lactobacillus delbrueckii is (Lactobacillus delbrueckii) DMLD-H1, and is preserved in the Guangdong Microbiological Culture Collection Center abbreviated as GDMCC on 16 April 2019, and the preservation number is GDMCC NO.60645. The strain disclosed by the invention is subjected to high-density culture, the viable count of prepared freeze-dried powder is as high as 10<10>-10<11>CFU / g, the moisture content of the freeze-dried powder is lower than 3%, the freeze-dried powder is not liable to agglomerate and good in rehydration properties, and has wide application such as preparation of ice cream, jelly, milk powder candies, solidification type yoghourt, stirring type yoghourt, yoghourt beverages or cheese and the like.

Description

technical field [0001] The invention relates to a Lactobacillus delbrueckii and a direct-throwing type freeze-dried powder of the Lactobacillus delbrueckii, in particular to a high-density culture method of the Lactobacillus delbrueckii and the obtained freeze-dried powder and its application. Background technique [0002] The high-density culture technology of lactic acid bacteria is also called high-density fermentation technology (high cell density culture, HCDC), generally refers to the culture of strains in a liquid environment, so that the cell density increases, more than 10 times the growth state or culture technology of conventional culture, Thereby, the purposes of increasing the fermentation density of the bacteria and increasing the specific productivity of the product can be achieved. The use of high-density culture technology can effectively improve production efficiency, thereby improving economic benefits. [0003] Direct-to-vat cultures (DVS) is a kind of c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/123A23G9/36A23C19/032A23L33/135C12R1/225
CPCC12N1/20A23C9/123A23G9/363A23C19/032A23L33/135C12R2001/225C12N1/205A23V2400/137
Inventor 刘冬梅彭鑫谷佳欣饶翔涂家霖肖弘毅
Owner SOUTH CHINA UNIV OF TECH
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