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Purpose and relevant medicine of ZEB1 (Zinc Finger E-Box-Binding Protein 1) gene

A gene and drug technology, applied in the direction of drug combination, gene therapy, medical preparations containing active ingredients, etc., can solve the problems of limited use and difficult treatment of patients, so as to alleviate osteoporosis, promote bone angiogenesis, and promote bone growth. generated effect

Pending Publication Date: 2019-12-31
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, for osteoporosis patients with other complications, such as postmenopausal osteoporosis patients with liver function damage, most of the drugs have certain toxic side effects, and the liver is responsible for detoxification, so the treatment for such patients more difficult
Overall, the side effects and other limitations of western medicines limit their clinical use

Method used

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  • Purpose and relevant medicine of ZEB1 (Zinc Finger E-Box-Binding Protein 1) gene
  • Purpose and relevant medicine of ZEB1 (Zinc Finger E-Box-Binding Protein 1) gene
  • Purpose and relevant medicine of ZEB1 (Zinc Finger E-Box-Binding Protein 1) gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The specific experimental method is as follows:

[0041] 1. Construction of transgenic mice

[0042] Will Zeb1 fl / fl Transgenic mice were continuously mated with mice expressing Tie2-Cre recombinase to obtain Tie2-Cre + / Zeb1 fl / fl Conditional knockout offspring mice, the ZEB1 gene in the mice is only in Tie2-Cre + specifically knocked out in endothelial cells.

[0043] 2. H.E. staining and immunofluorescence

[0044] H.E. staining: After the bone tissue was fixed, decalcified and dehydrated, it was coated with paraffin and sectioned. Paraffin sections were dewaxed in xylene, dehydrated with graded alcohol, stained with hematoxylin for 2-3 minutes, washed in tap water, stained with eosin for 1 minute, washed in tap water, dried and sealed.

[0045]Immunofluorescence: Immunofluorescence: After the bone tissue was quickly decalcified and dehydrated, embedded in OCT, frozen section, blocked with serum, incubated with primary antibody for 4 nights, incubated ...

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Abstract

The invention relates to a purpose and relevant medicine of a ZEB1 (Zinc Finger E-Box-Binding Protein 1) gene, and particularly relates to application of the ZEB1 gene to preparation of medicine for preventing and / or treating osteoporosis diseases, in particular to application of a ZEB1 gene lipidosome to preparation of medicine for preventing and / or treating osteoporosis diseases, so that the exogenous ZEB1 gene can be efficiently and specifically expressed in bone blood vessel endothelial cells. The ZEB1 gene lipidosome provided by the invention can obviously promote bone angiogenesis and promote osteogenesis. The effects on primary osteoporosis and secondary osteoporosis are obvious, and an obvious treatment effect is particularly achieved on postmenopausal osteoporosis. The ZEB1 gene provided by the invention can activate a Notch / Dll4 channel and can promote bone angiogenesis and osteogenesis, so that the bone loss is finally reduced; the osteoporosis process is inhibited; and theZEB1 gene lipidosome has low toxicity and is suitable for being further developed into gene medicine or medicine targets for preventing or treating osteoporosis.

Description

technical field [0001] The invention relates to the application of the ZEB1 gene and related medicines, and belongs to the technical field of biomedicine. Background technique [0002] Skeletal system disease is one of the most common chronic diseases in the world. It is a systemic metabolic bone disease characterized by low bone mass and microarchitectural degeneration of bone tissue, accompanied by increased bone fragility, decreased bone strength, and prone to fractures. . With the aging population, especially postmenopausal women, the incidence of osteoporosis and fracture complications is higher. Therefore, the prevention and treatment of osteoporosis has gradually become an important issue for the elderly, especially the postmenopausal women. At present, the drugs commonly used in the clinical treatment of osteoporosis mainly include: bone resorption inhibitors such as estrogen, estrogen receptor modulators, bisphosphonates, etc.; bone formation accelerators such as ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883A61K38/17A61K48/00A61P19/10
CPCC12Q1/6883A61K38/17A61K48/0033A61K48/005A61P19/10C12Q2600/158Y02A50/30
Inventor 吴照球傅蓉莫然
Owner CHINA PHARM UNIV
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