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Method of determining aspartame and alitame in food by reversed-phase liquid chromatography mixed standard sample adding method

A technology of reversed-phase liquid chromatography and aspartame, which is applied in the field of determination of aspartame and alitame in food by the mixed standard adding method of reversed-phase liquid chromatography, which can solve the different degrees of interference and side effects of the determination results , can not achieve simultaneous, simultaneous measurement, time-consuming and labor-intensive cost and other problems, to achieve the effect of low cost, high accuracy and time saving

Pending Publication Date: 2019-12-31
UNIV FOR SCI & TECH ZHENGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method mainly has the following disadvantages: (1) it is necessary to prepare 5-7 standard solutions of different concentrations, which is time-consuming, laborious and costly; Simultaneous and synchronous measurement cannot be realized. When the instrument conditions and environmental factors (such as temperature, humidity, atmospheric pressure, voltage, operating factors, instrument performance, etc.) inevitably fluctuate, there will be certain differences, which will have different degrees of influence on the measurement results , introducing a certain error; (3) The background background of the standard liquid and the liquid to be tested are different, the difference is large, and the degree of interference to the measurement results or the degree of possible side reactions are different
This method of determination needs to use the standard solution of each component to determine the respective quantitative correction factors and relative correction factors, the preparation workload is large, and when it cannot be guaranteed that all components in the sample have peaks or the correction factors for unknown peaks are missing When the normalization method cannot be used for quantitative analysis, the application is limited

Method used

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  • Method of determining aspartame and alitame in food by reversed-phase liquid chromatography mixed standard sample adding method
  • Method of determining aspartame and alitame in food by reversed-phase liquid chromatography mixed standard sample adding method
  • Method of determining aspartame and alitame in food by reversed-phase liquid chromatography mixed standard sample adding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Weigh about 5.000g (accurate to 0.001g) Coca-Cola carbonated beverage sample in a 50mL beaker, remove carbon dioxide on an ultrasonic oscillator, transfer all of it to a 25mL volumetric flask with water to make up the volume, mix well, and the measured components reach the concentration Balance, 4000r / min centrifuge for 5min, the concentration of aspartame and alitame in the supernatant and the lower layer are the same, centrifugation is to precipitate the particulate matter that may exist in the sample, take the supernatant and filter it with 0.45μm water system After membrane filtration, the liquid to be tested was obtained for chromatographic analysis.

[0070] Take two identical centrifuge tubes A and B, add 45 μL of 1 μg / mL aspartame and alitame mixed standard solution respectively, add no solution to be tested in A centrifuge tube, and dilute to 1 mL; add another 100 μL to B centrifuge tube Coca-Cola to be tested, the volume of the B centrifuge tube to the same sc...

Embodiment 2

[0099] Weigh about 1.000g (accurate to 0.001g) chrysanthemum crystal sample in a 50mL beaker, add 10mL water, extract by ultrasonic vibration for 20min, transfer the extract into a 25mL volumetric flask, add 10mL water to the residue and ultrasonically extract for 10min, Transfer the extract into the same 25mL volumetric flask, dilute to volume with water, mix well, the measured components reach concentration balance, centrifuge at 4000r / min for 5min, aspartame and alitame in the supernatant and lower layer The concentrations are the same, centrifugation is to precipitate the particulate matter that may exist in the sample, take the supernatant and filter it with a 0.45 μm water filter membrane to obtain the test solution for chromatographic analysis.

[0100] Take two identical centrifuge tubes A and B, add 45 μL of 1 μg / mL aspartame and alitame mixed standard solution respectively, add no solution to be tested in A centrifuge tube, and dilute to 1 mL; add another 100 μL to B ...

Embodiment 3

[0117] Weigh 5.000g (accurate to 0.001g) of yogurt sample into a 50mL centrifuge tube, add 10mL of ethanol to precipitate the protein in the yogurt sample, close the lid tightly, gently invert the centrifuge tube 5 times up and down (do not shake), and place After the centrifuge tube was mixed and vortexed for 10 seconds, let it stand for 1 minute, and centrifuged with a 4000r / min centrifuge for 5 minutes. Centrifugation is convenient for filtering a small amount of protein precipitation and other filter residues. Take the supernatant and filter it into a 25mL volumetric flask. 2+1) washing, after centrifugation, the supernatant was transferred to the same volumetric flask, then adjusted to 25mL with ethanol-water (2+1), mixed evenly, filtered through a 0.45μm organic filter membrane to obtain the test solution, and used analyzed by liquid chromatography.

[0118] Take two identical centrifuge tubes A and B, add 45 μL of 1 μg / mL aspartame and alitame mixed standard solution re...

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Abstract

According to the method disclosed by the invention, under the same liquid chromatography condition, an aspartame and alitame mixed standard solution and the aspartame and alitame mixed standard solution added with a to-be-tested solution are injected to a liquid chromatograph respectively, qualification is carried out based on whether component signals are increased or not under the same retentiontime condition, and whether alitame or aspartame is contained in a to-be-tested food is judged; and comparison between the peak appearance signal of the mixed standard solution with the peak appearance signal difference of the mixed standard solution added with the to-be-tested solution is carried out for quantification, and the contents of the alitame and the aspartame are measured. The method is high in working efficiency, low in cost, less in interference and high in accuracy.

Description

technical field [0001] The invention relates to the field of food testing, in particular to a method for measuring aspartame and alitame in food by a reversed-phase liquid chromatography mixed standard sample adding method. Background technique [0002] Aspartame and Alitame are commonly added sweeteners in food, and their sweetness is 200 times and 2000 times that of sucrose respectively. Compound use can synergize, reduce costs, improve taste, and improve the stability of the sweetness of sweeteners. It not only has high sweetness, good taste quality, but also has a long storage period and no calories. However, if the intake is excessive, there will be adverse effects such as nervous system disorder and damage to the human liver, so it is one of the indicators often measured in food. [0003] At present, methods for the determination of aspartame and alitame in food include liquid chromatography (GB5009.263-2016), liquid chromatography-mass spectrometry, ion chromatograph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/14
CPCG01N30/02G01N30/04G01N30/14G01N2030/042G01N2030/146
Inventor 高向阳
Owner UNIV FOR SCI & TECH ZHENGZHOU
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