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A two-phase aqueous extraction method of bletilla striata glucomannan

A technique for Bletilla striata glucomannan and stilbene glucomannan, which is applied in the field of glucomannan extraction, can solve the problems of low extraction efficiency, long time consumption, impure extraction components and the like, and achieves a short extraction period and high extraction rate. Effect

Active Publication Date: 2021-12-10
南京香林花雨生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, water extraction and alkaline water extraction have many disadvantages, such as long time-consuming, low extraction efficiency, and impure extraction components; enzymatic hydrolysis increases the complexity of the process, and the extraction process is likely to cause physical and chemical properties of glucomannan, Changes in product purity and biological activity

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  • A two-phase aqueous extraction method of bletilla striata glucomannan

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Effect test

Embodiment 1

[0033] A two-phase extraction method for bletilla striata glucomannan, comprising the following steps:

[0034] (1) After washing the bletilla striata, mix the bletilla striata with deionized water at a mass ratio of 1:4, put it in a homogenizer, and homogenize at 4°C, 13000rpm, for 5 minutes, with an interval of 1 minute, and the total homogenization time is 30 minutes , to prepare bletilla striata slurry;

[0035] (2) Centrifuge the Bletilla striata slurry in step (1) in a centrifuge at 4°C, 5000rpm, for 20min to obtain the supernatant;

[0036] (3) Establish an ethanol-dipotassium hydrogen phosphate two-phase system: the volume fraction of ethanol in the ethanol aqueous solution is 70%, the weight fraction of dipotassium hydrogen phosphate in the dipotassium hydrogen phosphate aqueous solution is 55%, and the ethanol aqueous solution and dipotassium hydrogen phosphate The volume ratio of the aqueous solution is 3:7; under the conditions of this volume ratio, it can not onl...

Embodiment 2

[0041] A two-phase extraction method for bletilla striata glucomannan, comprising the following steps:

[0042] (1) After washing the bletilla striata, mix the bletilla striata with deionized water at a mass ratio of 1:4, place it in a homogenizer, and homogenize at 6°C and 14,000rpm for 5 minutes, with an interval of 1 minute, and the total homogenization time is 25 minutes , to prepare bletilla striata slurry;

[0043] (2) Put the bletilla striata slurry in step (1) in a centrifuge, centrifuge at 6000 rpm for 15 minutes at 6°C to obtain the supernatant;

[0044] (3) Establish an ethanol-dipotassium hydrogen phosphate two-phase system: the volume fraction of ethanol in the ethanol aqueous solution is 70%, the weight fraction of dipotassium hydrogen phosphate in the dipotassium hydrogen phosphate aqueous solution is 55%, and the ethanol aqueous solution and dipotassium hydrogen phosphate The volume ratio of the aqueous solution is 3:7; under the conditions of this volume rati...

Embodiment 3

[0049] A two-phase extraction method for bletilla striata glucomannan, comprising the following steps:

[0050] (1) After washing the bletilla striata, mix the bletilla striata with deionized water at a mass ratio of 1:4, place it in a homogenizer, and homogenize at 10°C and 15,000rpm for 5 minutes, with an interval of 1 minute, and the total homogenization time is 25 minutes , to prepare bletilla striata slurry;

[0051] (2) Put the bletilla striata slurry in step (1) in a centrifuge, centrifuge at 10°C, 8000rpm, for 15min, and obtain the supernatant;

[0052] (3) Establish an ethanol-dipotassium hydrogen phosphate two-phase system: the volume fraction of ethanol in the ethanol aqueous solution is 70%, the weight fraction of dipotassium hydrogen phosphate in the dipotassium hydrogen phosphate aqueous solution is 55%, and the ethanol aqueous solution and dipotassium hydrogen phosphate The volume ratio of the aqueous solution is 3:7; under the conditions of this volume ratio, ...

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Abstract

The invention discloses a two-phase extraction method of bletilla striata glucomannan, relates to the technical field of glucomannan extraction, and comprises the following steps: (1) mixing bletilla striata with water and homogenizing it in a homogenizer to obtain bletilla striata slurry (2) centrifuging the bletilla striata slurry in step (1) to obtain a supernatant; (3) establishing an ethanol-dipotassium hydrogen phosphate two-phase system; (4) mixing the supernatant and ethanol in the step (2) ‑Dipotassium hydrogen phosphate two-phase system is placed in an ultrasonic countercurrent extraction device for extraction; (5) After the extract in step (4) is left to stand, the lower phase solution is removed and filtered, and the filtrate is respectively filtered through a molecular weight cut-off of 100K and 10K The hollow fiber ultrafiltration device is used for ultrafiltration separation. The beneficial effect of the present invention is that: the present invention has a short extraction period, a high extraction rate of glucomannan, and the prepared Bletilla striata glucomannan solution is colorless and transparent, and the content of glucomannan in the solid is 95.5-96.3%. Content 0.32‑0.35%.

Description

technical field [0001] The invention relates to the technical field of glucomannan extraction, in particular to an aqueous two-phase extraction method for bletilla striata glucomannan. Background technique [0002] Bletilla striata is the stem of Bletilla striata, which is bitter, sweet, astringent and slightly cold in nature. Bletilla striata is rich in glucomannan, and studies have shown that Bletilla striata glucomannan has biological activities such as anti-inflammatory and blood coagulation. As a natural polymer material, Bletilla striata glucomannan also has the characteristics of functional slow-release, local retention, self-degradability, non-irritating, non-toxic and side effects, cheap and easy to obtain, and its application in the process of drug preparation is becoming more and more popular. Pay attention to. Neutral glucomannan extracted from Bletilla striata has obvious ability to scavenge free radicals. With the increase of glucomannan concentration, its sc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/02
CPCC08B37/0087C08B37/0003
Inventor 邓辉陈存武韩邦兴张卫明陈乃东马世宏戴军宋程卫培培
Owner 南京香林花雨生物科技有限公司
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