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Rapid detection method for beta-cyclodextrin in water sample

A detection method, cyclodextrin technology, applied in the measurement of color/spectral properties, material analysis by observing the effect on chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem of expensive and repeatable instruments and equipment. problems such as poor performance, complex operation, etc., to achieve the effect of good application value, poor repeatability, and simple pre-processing

Inactive Publication Date: 2020-01-03
XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problems of poor stability, low anti-interference ability, poor repeatability, low accuracy, complicated operation and expensive equipment in the prior art, the purpose of the present invention is to provide a It is a rapid detection method for β-cyclodextrin in water bodies with good performance, wide detection range, low detection limit and strong anti-interference ability. -Demand for rapid detection and analysis of cyclodextrin

Method used

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  • Rapid detection method for beta-cyclodextrin in water sample
  • Rapid detection method for beta-cyclodextrin in water sample
  • Rapid detection method for beta-cyclodextrin in water sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] (1) Take the aforementioned water sample to be tested, in the water sample to be tested, adjust the pH value to 7.5 by dripping 0.1mol / L hydrochloric acid, and then pass through a 0.45 μm filter membrane to obtain a sample solution to be tested containing β-CD;

[0060] (2) Add the sample solution to be tested into a 25mL colorimetric tube, set to volume, add 0.5mL of iodine-potassium iodide chromogen (diluted 4 times before use), shake well, and let stand for 35min;

[0061] (3) Using a visible light spectrophotometer, within the wavelength range of 420nm, with a 1cm cuvette, with the reagent blank as a reference, the measured solution absorbance value is 0.079;

[0062] (4) according to the method in step (2) and (3), do reference with distilled water, draw the standard curve of β-CD, obtain standard curve regression equation y=0.0144x+0.0464, r 2 =0.9997, molar absorptivity ε=1.63×10 +4 ≥10 +4 , detection limit MDL = 0.694mg L -1 ;

[0063] (5) Substituting the a...

Embodiment 2

[0065] (1) Take the aforementioned water sample to be tested, in the water sample to be tested, adjust the pH value to 6.0 by dropwise adding 0.1mol / L hydrochloric acid, and then pass through a 0.45 μm filter membrane to obtain a sample solution to be tested containing β-CD;

[0066] (2) Add the sample solution to be tested into a 25mL colorimetric tube, set to volume, add 0.5mL of iodine-potassium iodide chromogen (diluted 4 times before use), shake well, and let stand for 25min;

[0067] (3) Using a visible light spectrophotometer, within the wavelength range of 465nm, with a 1cm cuvette, with the reagent blank as a reference, the measured solution absorbance value is 0.071;

[0068] (4) according to the method in step (2) and (3), do reference with distilled water, draw the standard curve of β-CD, obtain standard curve regression equation y=0.0107x+0.0479, r 2 =0.9988, molar absorptivity ε=1.21×10 +4 ≥10 +4 , detection limit MDL = 0.934mg L -1 ;

[0069] (5) Substitutin...

Embodiment 3

[0071] (1) Take the aforementioned water sample to be tested, and adjust the pH value to 8.5 by adding 0.1mol / L sodium hydroxide dropwise to the water sample to be tested, and then pass through a 0.45 μm filter membrane to obtain a sample to be tested containing β-CD solution;

[0072] (2) Add the sample solution to be tested into a 25mL colorimetric tube, set to volume, add 0.5mL of iodine-potassium iodide chromogen (diluted 4 times before use), shake well, and let stand for 60min;

[0073] (3) Using a visible light spectrophotometer, within the wavelength range of 400nm, use a 1cm cuvette, and use the reagent blank as a reference to measure the absorbance of the solution to 0.101;

[0074] (4) according to the method in step (2) and (3), do reference with distilled water, draw the standard curve of β-CD, obtain standard curve regression equation y=0.014x+0.0683, r 2 =0.9988, molar absorptivity ε=1.59×10 +4 ≥10 +4 , detection limit MDL = 0.714mg L -1 ;

[0075] (5) Subst...

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Abstract

The invention discloses a rapid detection method for beta-cyclodextrin in a water sample. The rapid detection method comprises the steps of taking a to-be-detected water sample, adjusting a pH value and filtering the to-be-detected water sample through a filter membrane to obtain a to-be-detected sample solution containing beta-CD; adding the to-be-detected sample solution and an iodine-potassiumiodide color-developing agent, shaking well and standing; determining a light absorption value of the solution by taking a reagent blank as reference; drawing a standard curve of the beta-CD by takingdistilled water as reference and obtaining a standard curve regression equation, sensitivity and a detection limit; and substituting the light absorption value of the to-be-detected sample solution into the standard curve regression equation, thereby obtaining a concentration value of the beta-CD in the solution. A spectrophotometry of taking iodine-potassium iodide mixed liquid as a color-developing agent has the advantages of being convenient and rapid to operate, simple in pretreatment, high in antijamming capability, relatively low in detection limit, large in detection range, easily available in instrument and reagent and the like, and is very suitable for the requirements of rapid detection and analysis of the beta-cyclodextrin in relevant water samples in the fields, such as pharmaceuticals, foods, cosmetics, textiles and environmental protection.

Description

technical field [0001] The invention belongs to the technical field of polymer compound analysis and detection, and in particular relates to a rapid detection method for β-cyclodextrin in water samples. Background technique [0002] β-cyclodextrin (abbreviated as β-CD), as a cyclic oligosaccharide with a hydrophilic outer edge and a hydrophobic cavity, can form host-guest inclusion complexes with many hydrophobic organic substances, and is widely used in pharmaceuticals (dissolving agent , slow and controlled release agents, irritation and side effect control agents, etc.), food (preservatives, deodorants, etc.), cosmetics (stabilizers, antioxidants, emulsifiers, etc.) and environmental protection (microbial degradation and photodegradation catalysts, eluents , solubilizers, etc.) and other fields of production and scientific research, and the efficient and convenient β-CD detection method is the basic pre-means to promote these works. At present, there is no national stand...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/78
CPCG01N21/31G01N21/78
Inventor 杨成建陈兴都刘伟苏含笑张军
Owner XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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