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Pretreatment method for promoting production of medium-chain fatty acid from abandonment activated sludge through anaerobic fermentation

A medium-chain fatty acid and anaerobic fermentation technology, which is applied in the field of solid waste pollution control and resource utilization, can solve the problems of a large amount of cost input and affect the production of medium-chain fatty acids, and achieve the effect of promoting degradation

Active Publication Date: 2020-01-07
TONGJI UNIV
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AI Technical Summary

Problems solved by technology

Due to the refractory nature of microbial cells, the anaerobic fermentation process of sludge will be limited by the dissolution of organic matter from sludge degradation, thereby affecting the production of medium-chain fatty acids
Therefore, some common pretreatment methods include heat treatment, mechanical treatment, acid-base treatment, and ultrasonic treatment, which can effectively destroy sludge flocs and cell walls and promote sludge degradation, but this requires a lot of cost input, so it needs to be explored A more cost-effective pretreatment method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The waste activated sludge in the secondary sedimentation tank of the urban sewage treatment plant is settled by gravity to obtain concentrated sludge. Add ammonium chloride solution to the concentrated sludge, adjust the pH=10, the temperature is 25°C, and control the initial FA concentration of the reaction to be 85mg / L.

[0036] The sludge after reacting for 24 hours was used as a fermentation substrate and added to multiple reactors with a working volume of 120ml, and then added inoculated sludge (rich in microorganisms capable of producing medium-chain fatty acids), growth medium and ethanol. Adjust the pH to 5.5, and pass high-purity nitrogen into the reactor for 1 minute to ensure an anaerobic environment, then cover it, and finally place it in a constant temperature shaking incubator at 170 rpm and 37°C. The concentration of medium-chain fatty acids was detected by gas chromatography every 2-3 days. After the reaction was stable, the concentration of n-hexanoic ...

Embodiment 2

[0038] The waste activated sludge in the secondary sedimentation tank of the urban sewage treatment plant is settled by gravity to obtain concentrated sludge. Add ammonium chloride solution to the concentrated sludge, adjust the pH=10, the temperature is 25°C, and control the initial FA concentration of the reaction to be 153mg / L.

[0039] The sludge after reacting for 24 hours was used as a fermentation substrate and added to multiple reactors with a working volume of 120ml, and then added inoculated sludge (rich in microorganisms capable of producing medium-chain fatty acids), growth medium and ethanol. Adjust the pH to 5.5, and pass high-purity nitrogen gas into the reactor for 1 minute to ensure an anaerobic environment, then cover it, and finally place it in a constant temperature shaking incubator at 170 rpm and 38°C. The concentration of medium-chain fatty acids was detected by gas chromatography every 2-3 days. After the reaction was stable, the concentration of n-hexa...

Embodiment 3

[0041] The waste activated sludge in the secondary sedimentation tank of the urban sewage treatment plant is settled by gravity to obtain concentrated sludge. Add ammonium chloride solution to the concentrated sludge, adjust the pH=10, the temperature is 25°C, and the initial FA concentration of the reaction is controlled to be 255mg / L.

[0042] The sludge after reacting for 24 hours was used as a fermentation substrate and added to multiple reactors with a working volume of 120ml, and then added inoculated sludge (rich in microorganisms capable of producing medium-chain fatty acids), growth medium and ethanol. Adjust the pH to 5.5, and pass high-purity nitrogen into the reactor for 1 minute to ensure an anaerobic environment, then cover it, and finally place it in a constant temperature shaking incubator at 170 rpm and 37°C. The concentration of medium-chain fatty acids was detected by gas chromatography every 2-3 days. After the reaction was stable, the concentration of n-hexa...

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Abstract

The invention relates to a pretreatment method for promoting production of medium-chain fatty acid from abandonment activated sludge through anaerobic fermentation. The pretreatment method comprises the steps of using the abandonment activated sludge from a secondary clarifier of a municipal wastewater treatment factory as a fermentation substrate, adding an ammonium chloride solution, controllingpH and temperature, performing free ammonia (FA) pretreatment, adding the pretreated sludge in an anaerobic reactor, adding electron donors, and performing anaerobic fermentation to produce the medium-chain fatty acid. Compared with the prior art, the pretreatment method has the advantages that a free ammonia pretreatment technique is used for promoting digesting of organic matters in the sludge,so that the yield of the medium-chain fatty acid during anaerobic fermentation can be increased, free ammonia can be obtained in an in-situ manner from fermentation liquid of the wastewater treatmentfactory, and on the basis that sludge recycling and harmless are increased, the method also has the advantages of being high in acid yield, safe, stable, simple to operate and the like.

Description

technical field [0001] The invention belongs to the field of solid waste pollution control and resource utilization, and in particular relates to a method in which free ammonia (FA) is used as a pretreatment technology to promote anaerobic fermentation of waste activated sludge to produce medium-chain fatty acids. Background technique [0002] With the acceleration of urbanization and the continuous development and expansion of urban sewage treatment plants, it is a serious problem to dispose of a large number of by-products waste activated sludge. The disposal cost of waste activated sludge accounts for about half of the total cost of sewage treatment plants, and in fact only about 35% is safely and properly disposed of, and the rest will still cause secondary pollution to the environment. But on the other hand, waste activated sludge is also a potential resource with huge output, which can be used as an organic substrate for anaerobic fermentation and energy recovery to co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/40
CPCC12P7/40Y02E50/30
Inventor 倪丙杰王云孙婧吴书林曹乐萱张子莎
Owner TONGJI UNIV
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