Micro-fluidic chip for culturing liver cancer slices and using method thereof

Abstract

The invention discloses a micro-fluidic chip for culturing liver cancer slices, which comprises a top layer and a culture layer, the culture layer comprises a micro-channel plate and a bottom plate, and the bottom plate, the micro-channel plate and the top layer are connected in sequence; the top layer is provided with a liquid inlet hole and a liquid outlet hole, the micro-channel plate is provided with an inflow channel, an outflow channel and a culture area, the inflow channel is communicated with the culture area, and the outflow channel is communicated with the culture area; the liquid inlet hole and the liquid outlet hole are circular holes; the cross sections of the inflow channel and the outflow channel are rectangular; wherein the culture area is a circular culture area; an inletof the inflow channel is connected with a first round hole with the same diameter as the liquid inlet, and an outlet of the outflow channel is connected with a second round hole with the same diameteras the liquid outlet; and threaded holes are formed in the top layer and the culture layer. The invention further discloses a using method of the micro-fluidic chip, the micro-fluidic chip can be used for in-vitro culture of liver cancer tissue slices, metabolic waste is discharged in time, and the growth condition of liver cancer tissue in a human body can be truly and accurately simulated.

Description

technical field [0001] The invention relates to a microfluidic chip, in particular to a microfluidic chip for culturing liver cancer slices and a method for using the chip. Background technique [0002] Primary hepatocellular carcinoma is one of the most common malignant tumors with high morbidity and mortality in the world. Epidemiological studies show that liver cancer ranks second among cancer killers worldwide. In China, the incidence of liver cancer ranks fourth, while the mortality rate ranks second only to lung cancer. However, there is currently a lack of effective treatment options. Therefore, it is imminent to construct a hepatocellular carcinoma model in vitro, further explore the pathogenesis of primary hepatocellular carcinoma, and develop new and effective therapeutic drugs. [0003] Hepatocellular carcinoma cell culture in vitro is a prerequisite for modeling liver structure and physiology, and is also a rapid means of obtaining information on drug metabolism...

AI Technical Summary

Problems solved by technology

This culture method requires a large number of hepatocytes, reagent materials, etc. At the same time, this static culture of metabolic waste is easy to accumulate and fails to simulate the three-dimensional growth state of hepatocytes in the human body.

In addition, only monoculture of HCC cells fails to take into account the interaction between HCC cells and other non-parenchymal cells in vivo

At present, animal experiments have a long experimental cycle, high cost, and involve animal ethics and moral issues, and the experimental data obtained on animals cannot fully reflect the real situation in the human body

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