A strain of Penicillium breenii strain and its application
A technology of penicillium and strains, which is applied in the direction of fungi, fungicides, chemicals for biological control, etc., and can solve the problems of unsatisfactory control effects of chemical pesticides and environmental pollution
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Embodiment 1
[0029] The Penicillium brefeldianum strain of the present invention, the Penicillium brefeldianum strain is PFLJ04, the preservation name is Penicillium brefeldianum (Penicillium brefeldianum), preserved in the General Microorganism Center (CGMCC) of China Microbial Culture Collection Management Committee, and the depository unit The address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. Date of deposit: August 15, 2019; Collection number: CGMCC No.18119.
[0030] The ITS gene sequence of the said Penicillium brazenii strain is the nucleotide sequence shown in SEQ ID No.1.
[0031] The Penicillium brescenium inoculum prepared by the Penicillium brescens strain of the present invention. Its active ingredient is at least one of the following (a) (b) (c):
[0032] (a) the fermented culture of described Penicillium breezii bacterial strain;
[0033] (b) the ultrasonic lysis supernatant of the obtained Penicillium brezhengii bacterial strain cells;
[0034]...
Embodiment 2
[0042] The difference between embodiment 2 and embodiment 1 is: the preparation method of the fermentation liquid of Penicillium brezhenii according to the present invention comprises the following steps: inoculating the described bacterial strain in the PDA culture medium dish, 28 ℃ upside-down culture for 7 days , activate the bacterial strain, use distilled water to prepare the activated bacterial strain into a spore suspension, count the spore concentration under a microscope, and the concentration of the spore suspension is: 1.0×10 6 cells / mL; inoculate 5% spore suspension in liquid medium PDB, culture at 28°C, 200r / min, for 7 days.
[0043] The PDA medium is composed of the following components: 200g of potatoes, 5g of glucose, 10g of agar, distilled to 1L of distilled water, natural pH, sterilized at 121°C for 30min; the PDB medium is composed of the following components: potatoes 200g, glucose 5g, dilute to 1L distilled water, pH 8, sterilize at 121°C for 30min.
[00...
Embodiment 3
[0046] The difference between embodiment 3 and embodiment 1 is: the preparation method of the fermentation liquid of Penicillium brezhenii according to the present invention comprises the following steps: inoculating the described bacterial strain in the PDA culture medium dish, 28 ℃ upside-down culture 7 days , activate the bacterial strain, use distilled water to prepare the activated bacterial strain into a spore suspension, count the spore concentration under a microscope, and the concentration of the spore suspension is: 8.0×10 4 Individuals / mL; 5% spore suspension was inoculated in liquid medium PDB, cultured at 28° C., 200 r / min, for 9 days.
[0047] The PDA medium is composed of the following components: 180g of potatoes, 15g of glucose, 20g of agar, distilled to 1L of distilled water, natural pH, sterilized at 121°C for 30min; the PDB medium is composed of the following components: potatoes 100g, glucose 20g, dilute to 1L distilled water, pH 7, sterilize at 121°C for ...
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