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Stable xylidyl blue method serum magnesium detection kit and preparation method thereof

A xylidine blue and detection kit technology, applied in the field of clinical diagnosis, can solve the problems of calcium ion interference, stability influence, etc., and achieve the effects of strong anti-calcium ion interference ability, good stability, and improved anti-interference ability.

Inactive Publication Date: 2020-01-10
沈阳医陆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a stable xylene blue method serum magnesium detection kit and its preparation for the problem that the xylene blue method detects serum magnesium ion reagents easily interfered by calcium ions and easily absorbs carbon dioxide, resulting in affected stability. method

Method used

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  • Stable xylidyl blue method serum magnesium detection kit and preparation method thereof
  • Stable xylidyl blue method serum magnesium detection kit and preparation method thereof
  • Stable xylidyl blue method serum magnesium detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] In this implementation, the components in each liter of reagent in the kit are:

[0022]

[0023] The balance is purified water;

[0024] Specific configuration method:

[0025] First, the material is weighed. The weighed amount of diphenylmethylene blue was 0.3250g, the weighed amount of EGTA was 0.1626g, the weighed amount of Tween 20 was 2.6014g, the weighed amount of sodium bicarbonate was 0.5462g, and the weighed amount of sodium carbonate was 6.2011g.

[0026] Next, stir. Into the reaction kettle containing 800ml purified water, add the above-mentioned weighed sodium bicarbonate, sodium carbonate, masking agent, surfactant and xylidine blue in sequence, and set the rotation speed to 300r / min for stirring. Each addition of material must be completely dissolved before adding the next material. After all materials are completely dissolved, dilute to 1L with purified water.

[0027] Finally, the reagents are filtered and aliquoted. After the reagent is filter...

Embodiment 2

[0029] In this implementation, the components in each liter of reagent in the kit are:

[0030]

[0031]

[0032] The balance is purified water;

[0033] Specific configuration method:

[0034] First, the material is weighed. The weighed amount of diphenylmethylene blue is 0.0501g, the weighed amount of EGTA is 0.0252g, the weighed amount of Tween 20 is 0.4012g, the weighed amount of sodium bicarbonate is 0.0841g, and the weighed amount of sodium carbonate is 0.9541g.

[0035] Next, stir. Add sodium bicarbonate, sodium carbonate, masking agent, surfactant and xylidine blue to the reaction kettle containing 800ml of purified water in sequence, and set the rotation speed at 300r / min for stirring. Each addition of material must be completely dissolved before adding the next material. After all materials are completely dissolved, dilute to 1L with purified water.

[0036] Finally, the reagents are filtered and aliquoted. After the reagent is filtered through a peristal...

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Abstract

The invention relates to a clinical diagnosis technology and particularly relates to a stable xylidyl blue method serum magnesium detection kit and a preparation method thereof. The kit comprises thefollowing components including 0.05 to 0.24 g / L of xylidyl blue, 0.025 to 0.059 g / L of a masking agent, 0.4 to 1.2 g / L of a surfactant, 1.0 to 1.5 g / L of an alkaline buffer solution with a pH value of10.2 to 11.3, and the balance of water. Sodium bicarbonate, sodium carbonate, the masking agent, the surfactant and the xylidyl blue are sequentially added into water and are dissolved while being stirred, and the volume is fixed after dissolving, and therefore, a reagent is obtained. The kit of the invention has the advantages of high calcium ion interference resistance, good stability, wide linear range, easy operation and convenient storage and transportation, and the kit is suitable for a semi-automatic or full-automatic biochemical analyzer and has high clinical application value.

Description

technical field [0001] The invention relates to clinical diagnosis technology, in particular to a stable xylene blue method serum magnesium detection kit and a preparation method thereof. Background technique [0002] Magnesium is an important cation in the human body, which can activate the catalytic function of various enzymes and participate in the physiological and biochemical reactions of the human body. The determination of serum magnesium ion content has been used as an important basis for the diagnosis of patients' diseases in clinical practice. With the development of diagnostic technology, there are more and more methods for detecting serum magnesium ion content in clinical application. At this stage, the main methods used in clinical testing include enzymatic method, atomic absorption spectrophotometry, colorimetric method and so on. The accuracy of atomic absorption spectrophotometry and enzymatic method is the most clinically recognized, but the detection equi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/01G01N21/31
CPCG01N21/01G01N21/3103
Inventor 兰玉勇刘丽杨丽丽
Owner 沈阳医陆生物科技有限公司