Cellular injury model preparation method based on ultraviolet irradiation
A cell damage and ultraviolet technology, applied in the field of cell experiments, can solve the problems of the large difference between the model and the human skin cell model and the low preparation success rate, and achieve the effect of improved survival rate, accurate experimental data and good proliferation.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0021] A method for preparing a cell damage model based on ultraviolet radiation, the method comprising the following steps:
[0022] S1: Cut out the normal human skin tissue in the clinical operation, and disinfect it with povidone iodine. After the disinfection is completed, use 0.25% trypsin digestion solution to digest the skin tissue, disperse the tissue into single cells, and then put the cell solution in a suitable medium , place the culture medium in an incubator to allow these primary cells to grow and proliferate;
[0023] S2: After about 24 hours, the primary cells adhered to the wall and overgrown with the medium, then digested with 0.25% trypsin digestion solution, replaced with new medium and seeded on multiple six-well plates, the seeding density of the six-well plates About 5X10 4 / ml, each six-well plate after inoculation was placed in an incubator and cultured until it adhered to the wall, and then replaced with a new medium for further experiments. Multiple...
Embodiment 2
[0031] A method for preparing a cell damage model based on ultraviolet radiation, the method comprising the following steps:
[0032] S1: Cut out the normal human skin tissue in the clinical operation, and disinfect it with povidone iodine. After the disinfection is completed, use 0.25% trypsin digestion solution to digest the skin tissue, disperse the tissue into single cells, and then put the cell solution in a suitable medium , place the culture medium in an incubator to allow these primary cells to grow and proliferate;
[0033] S2: After about 24 hours, the primary cells adhered to the wall and overgrown with the medium, then digested with 0.25% trypsin digestion solution, replaced with new medium and seeded on multiple six-well plates, the seeding density of the six-well plates About 4X10 4 / ml, each six-well plate after inoculation was placed in an incubator and cultured until it adhered to the wall, and then replaced with a new medium for further experiments. Multiple...
Embodiment 3
[0041] A method for preparing a cell damage model based on ultraviolet radiation, the method comprising the following steps:
[0042]S1: Cut out the normal human skin tissue in the clinical operation, and disinfect it with povidone iodine. After the disinfection is completed, use 0.25% trypsin digestion solution to digest the skin tissue, disperse the tissue into single cells, and then put the cell solution in a suitable medium , place the culture medium in an incubator to allow these primary cells to grow and proliferate;
[0043] S2: After about 24 hours, the primary cells adhered to the wall and overgrown with the medium, then digested with 0.25% trypsin digestion solution, replaced with new medium and seeded on multiple six-well plates, the seeding density of the six-well plates About 6X10 4 / ml, each six-well plate after inoculation was placed in an incubator and cultured until it adhered to the wall, and then replaced with a new medium for further experiments. Multiple ...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More