Thin layer detection method for quickly identifying honeysuckle and mountain honeysuckle

A technology of thin-layer detection and honeysuckle, which is applied in the field of plant identification, can solve the problems of long time, high cost, and low reliability, and achieve the effect of simple method, obvious contrast, and strong specificity

Active Publication Date: 2020-01-14
SHAANXI JIAHE PHYTOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0020] The purpose of the present invention is to solve the disadvantages of low reliability, high cost and long time in the four methods for identifying honeysuckle and mountain silver flower in the background technology, and provide a new method for identifying honeysuckle and mountain silver flower method

Method used

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  • Thin layer detection method for quickly identifying honeysuckle and mountain honeysuckle
  • Thin layer detection method for quickly identifying honeysuckle and mountain honeysuckle
  • Thin layer detection method for quickly identifying honeysuckle and mountain honeysuckle

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Embodiment 1

[0075] A thin-layer detection method for rapidly distinguishing honeysuckle and mountain silver flower, comprising the following steps:

[0076] 1) Prepare reference substance solution and sample solution

[0077] 1.1) Take an appropriate amount of chlorogenic acid, luteolin, honeysuckle saponin B and Dipsacus saponin B, dissolve in methanol to make a reference solution with a concentration of 1 mg / ml, and number them in sequence as No. 1, No. 2, No. 3, No. 4.

[0078] 1.2) Take appropriate amount of honeysuckle (including honeysuckle reference medicinal material and honeysuckle test sample) and mountain silver flower (including mountain silver flower control medicinal material and mountain silver flower test sample), crush them and pass through an 80-mesh sieve, and place them in a Daisy triangular flask Among them, there are four groups, and they are numbered: No. 5 is honeysuckle control medicinal material, No. 6 is mountain silver flower control medicinal material, No. 7 ...

Embodiment 2

[0098] The difference with Embodiment 1 is:

[0099] 1.1) Take appropriate amount of chlorogenic acid, luteolin, Lonicerae saponin B and Dipsapacea saponin B respectively, and dissolve them in methanol to make a 0.8 mg / ml reference solution;

[0100] In step 1.3), add respectively to the four groups of Erlenmeyer flasks in Step 1.2) the amount of 8 times the amount of the sample in the bottle, and the mass concentration is 70% ethanol, and perform ultrasonic treatment for 45 minutes, take out the Erlenmeyer flask and put it at room temperature;

[0101] In step 2), the distance between the origin of sample application and the lower edge of the silica gel G thin-layer plate is 1.5cm;

[0102] In step 3), the specification of the double-slot expanded steel is 10×20cm; and the developing agent is presaturated in the expanding cylinder for 20 minutes;

[0103] In step 4), when the leading edge of the developing agent goes up to the edge of the silica gel G thin-layer plate and is...

Embodiment 3

[0107] The difference with Embodiment 1 is:

[0108] 1.1) Take an appropriate amount of chlorogenic acid, luteolin, Lonicerae saponin B and Dipsacus saponin B respectively, and dissolve them in methanol to make a 2 mg / ml reference solution;

[0109] In step 1.3), add ethanol with 10 times the sample mass and a mass concentration of 95% to the four groups of conical flasks in step 1.2), perform ultrasonic treatment for 30 minutes, take out the conical flask and place it at room temperature;

[0110] In step 2), the distance between the origin of sample application and the lower edge of the silica gel G thin-layer plate is 1.2cm;

[0111] In step 4), when the leading edge of the developing agent goes up to the edge of the silica gel G thin-layer plate and is 15 cm away from the origin of sample application, take out the silica gel G thin-layer plate;

[0112] In step 5.1), after spraying with a natural color developer, place it on an electric heating plate, heat it at 105°C for...

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Abstract

The invention provides a thin layer detection method for quickly identifying honeysuckle and mountain honeysuckle, which solves the problems of low reliability, high cost, and long time in the existing four methods for identifying honeysuckle and mountain honeysuckle. In this method the characteristic representative ingredients of honeysuckle and mountain honeysuckle are selected to identify the two, including the following steps: 1) preparation of reference solution and sample solution; 2) thin layer spot sample; 3) preparation of developing agent; 4) deployment; 5) color development and inspection. This thin layer detection method is simple, fast, high sensitivity, and strong specificity, the contrast between honeysuckle and mountain honeysuckle is obvious, the results are intuitive andeasy to identify and distinguish, and it provides a reliable identification method to ensure the distinction between honeysuckle and mountain honeysuckle.

Description

technical field [0001] The invention belongs to the field of plant identification, and in particular relates to a thin-layer detection method for quickly identifying honeysuckle and mountain silver flowers. Background technique [0002] The 2015 edition of "Chinese Pharmacopoeia" stipulates that honeysuckle is the dry flower bud or flower with early opening of Lonicera japonica Thunb.; it is harvested and dried before the flower blooms in early summer; it is mainly produced in Henan, Shandong, Guangxi, Hunan, Guangdong and other places. It is a traditional Chinese medicine commonly used clinically, known as "penicillin in traditional Chinese medicine". Mountain silver flower is Lonicera macranthoides Hand.-Mazz., Lonicera hypoglauca Miq., Lonicera confusa D C. or Lonicera fulvotomentosa Hsu et S.C.Cheng. Dried buds or flowers with early opening; harvested in early summer before the flowers open, and dried. [0003] It can be seen from this that, although both Lonicera and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/94G01N30/95G01N1/28G01N1/34
CPCG01N1/286G01N1/34G01N30/90G01N30/94G01N30/95
Inventor 高娟屈花左志超惠战峰惠玉虎
Owner SHAANXI JIAHE PHYTOCHEM
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