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Preparation method and application of macadamia nut green skin extract

An extract and green peel technology, applied in the field of preparation of macadamia nut green peel extract, can solve the problems of complicated operation process, hinder comprehensive utilization of macadamia nut green peel resources, unsuitable for industrial production, etc., and achieve the effect of simple extraction process

Pending Publication Date: 2020-01-17
JIANGCHENG ZHONGAO AGRI TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, since green bark contains many types of phenolic compounds and other substances, if one wants to separate and obtain one or more phenolic compounds, a relatively complicated operation process is required, such as multiple extractions and column purification and separation.
The extraction and separation of polyphenols from the green bark of macadamia nuts in the prior art is not suitable for industrial production, thus hindering the comprehensive utilization of resources of green bark of macadamia nuts

Method used

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  • Preparation method and application of macadamia nut green skin extract

Examples

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Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: Preparation example of macadamia nut green bark extract

[0042] The preparation steps of macadamia nut green bark extract are as follows:

[0043] Step (1): Take fresh macadamia nut green skin, wash it, dry it in the shade, crush it and pass it through 40 meshes to obtain green skin powder;

[0044] Step (2): Add 20ml of water to every 1g of green bark powder, and then add cellulase (aladdin, C298989, CAS No.: 9012-54-8) to obtain enzymatic hydrolysis system I, and carry out the first enzymatic hydrolysis reaction. In the enzymatic hydrolysis system I, the mass concentration of cellulase was 3%, the enzymatic hydrolysis reaction time was 30min, and the temperature was 50°C.

[0045] After the first enzymatic hydrolysis reaction, the pH value was adjusted to 9.5, and then alkaline protease (McLean, P824138, CAS No.: 9014-01-1) was added to obtain enzymatic hydrolysis system II for the second enzymatic hydrolysis reaction. The mass concentration of alkali...

Embodiment 2

[0051] Example of preparation of macadamia nut green bark extract

[0052] The preparation steps of macadamia nut green bark extract are as follows:

[0053] Step (1): Take fresh macadamia nut green skin, wash it, dry it in the shade, crush it and pass it through 40 meshes to obtain green skin powder;

[0054] Step (2): Add 15ml of water to every 1g of green bark powder, and then add cellulase (aladdin, C298989, CAS No.: 9012-54-8) to obtain enzymatic hydrolysis system I, and carry out the first enzymatic hydrolysis reaction. In the enzymatic hydrolysis system I, the mass concentration of cellulase is 2%, the enzymatic hydrolysis reaction time is 30min, and the temperature is 40°C.

[0055] After the first enzymatic hydrolysis reaction, the pH value was adjusted to 10.5, and then alkaline protease (McLean, P824138, CAS No.: 9014-01-1) was added to obtain enzymatic hydrolysis system II for the second enzymatic hydrolysis reaction. The mass concentration of alkaline protease i...

Embodiment 3

[0060] Embodiment 3: Preparation example of macadamia nut green bark extract

[0061] The preparation steps of macadamia nut green bark extract are as follows:

[0062] Step (1): Take fresh macadamia nut green skin, wash it, dry it in the shade, crush it and pass it through 40 meshes to obtain green skin powder;

[0063] Step (2): Add 18ml of water to every 1g of green bark powder, and then add cellulase (aladdin, C298989, CAS No.: 9012-54-8) to obtain enzymatic hydrolysis system I, and carry out the first enzymatic hydrolysis reaction. In the enzymatic hydrolysis system I, the mass concentration of cellulase was 4%, the enzymatic hydrolysis reaction time was 25min, and the temperature was 55°C.

[0064] After the first enzymatic hydrolysis reaction, the pH value was adjusted to 10.0, and then alkaline protease (McLean, P824138, CAS No.: 9014-01-1) was added to obtain enzymatic hydrolysis system II for the second enzymatic hydrolysis reaction. The mass concentration of alkal...

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Abstract

The invention belongs to the field of extraction of plant natural products, and particularly relates to a preparation method and application of a macadamia nut green skin extract. The preparation method comprises the steps of cellulase hydrolysis and protease enzymolysis, and then phenolic glycoside compounds with high utilization value such as arbutin, tofu glucoside and gastrodin in macadamia nut green skins are extracted by an ultrasonic extraction method, and finally, the green skin extract is obtained through degreasing treatment and decolorization treatment. The green skin extract obtained by the preparation method and the phenolic glycoside compounds such as the arbutin, the tofu glucoside and the gastrodin are high in content, the extraction process is simple, complex extraction, purification and separation operations are not needed, reagents, raw materials and the like used in the process are easy to obtain, and the cost is low. The preparation method can be applied to preparation of health-care foods and cosmetics containing the arbutin, the tofu glucoside, the gastrodin and the like.

Description

technical field [0001] The invention belongs to the field of plant natural product extraction, and in particular relates to a preparation method and application of macadamia nut green bark extract. Background technique [0002] The fruit of macadamia nut (Macadamia integrifolia F.Muell, also known as macadamia nut) includes green skin, brown seed coat and milky white kernel. The green peel is the peel of the macadamia nut fruit, which accounts for 45-60% of the fresh weight of the fruit. It belongs to the discarded part of the fruit, and only a small amount is used as manure and animal feed. The large amount of active substances in the green peel has not been rationally developed. use. [0003] Studies have found that macadamia nut green bark contains a large amount of phenolic compounds, which have the functions of antibacterial, anti-oxidation, preventing dental caries, anti-tumor, etc., and also have a certain fragrance and whitening effect. The polyphenols of macadamia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105A23L29/00A61K8/9789A61Q19/02A61Q19/00
CPCA23L33/105A23L29/06A61K8/9789A61Q19/02A61Q19/004A23V2002/00A23V2200/318A23V2250/21
Inventor 徐斌施蕊万举河卢淼马宁李彪
Owner JIANGCHENG ZHONGAO AGRI TECH DEV
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