Rabbit coccidiosis live vaccine and preparation method and application thereof

A rabbit coccidiosis and live vaccine technology, applied in vaccines, veterinary vaccines, pharmaceutical formulations, etc., can solve the problems of affecting production performance, economic benefits of rabbit industry, severe diarrhea, etc., and achieve high immune protection efficacy and safety. The effect of disease reduction and long duration of immunity

Active Publication Date: 2020-01-17
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, Eimeria large, Eimeria medium, Eimeria intestinalis and Eimeria perforatus are the main insect species infected and popular in rabbit farms, and the

Method used

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  • Rabbit coccidiosis live vaccine and preparation method and application thereof
  • Rabbit coccidiosis live vaccine and preparation method and application thereof
  • Rabbit coccidiosis live vaccine and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The breeding method (1) of embodiment 1 coccidian prematurity weakened strain

[0039] The present embodiment provides a method for breeding a precoccidia weakened precoccidia strain, comprising the steps of:

[0040] (1) First inoculation: the coccidian strain to be weakened was injected with 1.0×10 5 Inoculation dose of sporulated oocysts / rabbit Oral inoculation of clean grade (no coccidia infection) New Zealand white rabbits;

[0041] (2) Repeated inoculation: 12 hours after the first inoculation, the coccidia strains to be weakened were treated with 5.0×10 5 The inoculation dose of sporulated oocysts / rabbit was orally inoculated again; 24 hours after the first inoculation, the coccidia strain to be weakened was injected with 1.0×10 6 The inoculation dose of sporulated oocysts / rabbit is orally inoculated once;

[0042] (3) Harvest the earliest discharged oocysts, and carry out subculture amplification after sporulation is completed under suitable conditions.

Embodiment 2

[0043] The breeding method (2) of embodiment 2 coccidian prematurity weakened strain

[0044] The present embodiment provides a method for breeding a precoccidia weakened precoccidia strain, comprising the steps of:

[0045] (1) First inoculation: the coccidian strain to be weakened was injected with 1.0×10 5 Inoculation dose of sporulated oocysts / rabbit Oral inoculation of clean grade (no coccidia infection) New Zealand white rabbits;

[0046] (2) Repeated inoculation: 24 hours after the first inoculation, the coccidia strains to be weakened were treated with 5.0×10 5 The inoculation dose of sporulated oocysts / rabbit was orally inoculated once again; 36 hours after the first inoculation, the coccidia strain to be weakened was injected with 1.0×10 6 The inoculation dose of sporulated oocysts / rabbit is orally inoculated once;

[0047] (3) Harvest the earliest discharged oocysts, and carry out subculture amplification after sporulation is completed under suitable conditions. ...

Embodiment 3

[0048] The breeding method of embodiment 3 coccidian precocious and weakened strains (3)

[0049] The present embodiment provides a method for breeding a precoccidia weakened precoccidia strain, comprising the steps of:

[0050] (1) Inoculation for the first time: the coccidia strain to be weakened was injected with 2.0×10 5 Inoculation dose of sporulated oocysts / rabbit Oral inoculation of clean grade (no coccidia infection) New Zealand white rabbits;

[0051] (2) Repeated inoculation: 12 hours after the first inoculation, the coccidia strain to be weakened was treated with 1.0×10 6 The inoculation dose of sporulated oocysts / rabbit was orally inoculated again; 24 hours after the first inoculation, the coccidia strain to be weakened was injected with 2.0×10 6 The inoculation dose of sporulated oocysts / rabbit is orally inoculated once;

[0052] (3) Harvest the earliest discharged oocysts, and carry out subculture amplification after sporulation is completed under suitable con...

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Abstract

The invention relates to the technical field of animal coccidiosis vaccines, in particular to a rabbit coccidiosis live vaccine and a preparation method and application thereof, and provides a rabbitcoccidiosis live vaccine composition which comprises precocious attenuated strains of Eimeria magna, Eimeria intestinalis and Eimeria media and precocious attenuated strain of Eimeria perforans. The rabbit coccidiosis live vaccine has high immunoprotective efficacy and safety, can effectively prevent and treat rabbit coccidiosis and reduce or avoid using of coccidiostats, can remarkably reduce unfavorable impact of vaccine inoculation on growth and production performance like rabbit weight gain and has great economic benefit and wide application prospect. The invention further provides a breeding method of coccidium precocious attenuated strains. The breeding method can effectively shorten breeding period of precocious strains and improve breeding efficiency of the coccidium precocious attenuated strains, obtained precocious strains are stable in hereditary character and high in immunogenicity, and pathogenicity is remarkably lowered.

Description

technical field [0001] The invention relates to the technical field of animal coccidiosis vaccines, in particular to a live rabbit coccidiosis vaccine and its preparation method and application. Background technique [0002] Antibiotics and anticoccidial drugs have been used as livestock and poultry feed additives for a long time, and they are the main means to ensure the health of livestock and poultry under the intensive farming model. With the frequent occurrence of drug residues and drug-resistant bacteria (worm) strains and threats to the environment and human health, the strategy of using drugs to prevent and control animal diseases has gradually exposed its drawbacks. Outbreaks of rabbit coccidiosis can cause large-scale death of rabbits; chronic and subclinical coccidiosis infection can lead to decreased animal production performance and reduced economic benefits. The continuous occurrence of coccidiosis is the main reason why many rabbit farms have low economic ben...

Claims

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Application Information

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IPC IPC(8): A61K39/012A61P33/02
CPCA61K39/012A61K2039/542A61K2039/552A61P33/02
Inventor 索勋刘贤勇索静霞李超顾小龙汤新明汪运舟崔玉娟陶鸽如王丹廖琴成霞罗光琴邓梦青吕艳丽
Owner CHINA AGRI UNIV
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