Double-stranded RNA for improving disease resistance of eriocheir sinensis and application thereof

A Chinese mitten crab, sense strand technology, applied in the direction of DNA/RNA fragment, application, recombinant DNA technology, etc., can solve the problems of reduced promoter activity, reduced expression of LvMMP-2, etc., to improve disease resistance, reduce Mortality, effect of high commercial application value

Active Publication Date: 2020-01-17
TIANJIN NORMAL UNIVERSITY
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Problems solved by technology

At the same time, further studies have found that the proto-oncogene c-Jun of Litopenaeus vannamei can enhance the promoter activity of LvMMP-2 and promote its transcription; while reducing the expression of LvMMP-2, the shrimp antimicrobial peptide 2 (PEN2) and β- The promoter activity of defensins was significantly reduced

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  • Double-stranded RNA for improving disease resistance of eriocheir sinensis and application thereof
  • Double-stranded RNA for improving disease resistance of eriocheir sinensis and application thereof
  • Double-stranded RNA for improving disease resistance of eriocheir sinensis and application thereof

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[0027] Further illustrate technical scheme of the present invention below in conjunction with specific embodiment

[0028] This research was supported by the National Key Research and Development Program "Blue Granary Science and Technology Innovation" project "Important Farmed Shrimp and Crab Germplasm Creation and Healthy Seed Breeding" project "Shrimp and Crab New Germplasm Creation Technology Platform" project (2018YFD0901301); Tianjin talent The development of special support programs for high-level innovation and entrepreneurship team projects is supported by Tianjin Aquaculture Industry Technology System Innovation Team (ITTFRS2017007), and Tianjin University Innovation Team Construction (TD13-5076).

[0029] The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the following examples, unless otherwise specified, were purchased from conventional biochemical reagent stores. % in the following e...

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Abstract

The invention discloses double-stranded RNA for improving the disease resistance of eriocheir sinensis and application thereof. The RNAi vector of the important gene EsMMP-14 participated in natural immunity, cell proliferation and tissue generation of the eriocheir sinensis is constructed, and the vector can be used for improving the disease resistance of the eriocheir sinensis, and has the characteristics of environmental friendliness and higher commercial application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a double-stranded RNA for improving the disease resistance of Chinese mitten crabs and an application thereof. Background technique [0002] Matrix metalloproteinase is a calcium ion-dependent zinc ion-containing endopeptidase. Due to its internal Zn 2+ , the active site contains metalloprotease residues, hence the name matrix metalloprotease. MMPs were first discovered by Gross and Lapier in 1962 when tadpoles docked their tails, a protease capable of degrading collagen fibers. With the in-depth research on the MMPs family, more than 50 members of the MMPs family have been discovered. Members of the MMPs family have multiple functions and are involved in the degradation of cell-to-cell connections, cell-basement membrane connections, basement membrane and extracellular matrix components, such as MMP-1 involved in the degradation of extracellular matrix (ECM) ; MMP-7 c...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/85C12N15/12
CPCC12N15/113C07K14/43509C12N2310/14Y02A40/81
Inventor 李冉孟庆昊孙金生
Owner TIANJIN NORMAL UNIVERSITY
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