Nucleic acid detection device and detection method

A detection device and nucleic acid technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of harsh reagent storage conditions, easy attenuation, and unstable fluorescence signals, and achieve controllable cycle number and reaction speed Fast, stable signal output effect

Inactive Publication Date: 2020-01-17
TDK CORPARATION
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, PCR microanalysis systems including the above-mentioned patent publications still use fluorescent dyes and fluorescent signal analysis systems to detect and analyze the amplified DNA, and the fluorescent signal is unstable and easy to decay, and its reagent storage relatively harsh conditions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nucleic acid detection device and detection method
  • Nucleic acid detection device and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A nucleic acid detection device according to an embodiment of the present invention, which includes a sample processor and a magnetic-sensitive detector; the structure of the sample processor in this embodiment is as follows figure 1 As shown, it includes a reagent chamber 1, a plurality of PCR reaction microchannels 2 arranged in parallel, a capture chip storage chamber 41, a DNA modification magnetic bead storage chamber 42 and a cleaning solution storage chamber 43;

[0043] The reagent chamber 1 is provided with a reagent inlet and a reagent outlet; the top of the first PCR reaction microchannel 2 communicates with the reagent outlet of the reagent chamber 1 through the first microchannel 71, and two adjacent PCR reaction microchannels 2 pass through the second microchannel 71. The microchannel 72 is connected end to end, so that a plurality of PCR reaction microchannels 2 are connected in series to form a circuitous flow path, and the first microchannel 71 is provid...

Embodiment 2

[0068] A nucleic acid detection device according to an embodiment of the present invention differs from Embodiment 1 only in the reagent chamber 1 in the sample processor. The sample processor of the nucleic acid detection device in this embodiment is as follows: figure 2 As shown, the reagent chamber 1 does not include a DNA extraction chamber 13 with a built-in DNA extraction solution, but the reagent chamber 1 of this embodiment also includes an RNA extraction chamber 14 with a built-in RNA extraction solution and a reverse transcription reagent storage chamber 15. The storage chamber 15 communicates with the reagent inlets of the RNA extraction chamber and the DNA storage chamber 11 to be tested respectively.

[0069] Using the nucleic acid detection device of this embodiment, RNA can be extracted and RNA assayed. The method of using the nucleic acid detection device of this embodiment is different from that of Example 1 only in step (1). When using the nucleic acid detec...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a nucleic acid detection device and detection method. The device comprises a sample processor and a magnetic sensitive detector, wherein the sample processor comprises a reagent chamber, a plurality of PCR reaction microchannels which are arranged side by side, a catching chip storage chamber, a DNA modification magnetic bead storage chamber and a cleaning liquid storage chamber, wherein the top end of the first PCR reaction microchannel communicates with a reagent outlet through a first microchannel, and every two adjacent PCR reaction microchannels are in communication in heads and tails through a second microchannel; 3 temperature control devices are sequentially arranged on each PCR reaction microchannel from top to bottom; the inlet of the catching chip storage chamber communicates with the bottom end of the last PCR reaction microchannel through a third microchannel, communicates with the DNA modification magnetic bead storage chamber through a fourth microchannel, and communicates with the cleaning liquid storage chamber through a fifth microchannel; and a magnetic sensor of the magnetic sensitive detector is used for sensing and catching DNA modification magnetic beads in the magnetic sensitive detector and converting the magnetic signals of the DNA modification magnetic beads into electrical signals. When being used for detecting nucleic acid,the device disclosed by the invention is high in reaction speed and stable in signal output.

Description

technical field [0001] The invention relates to a detection device and a detection method, in particular to a nucleic acid detection device and a detection method. Background technique [0002] Polymerase chain reaction (PCR) is a molecular biology technique for amplifying specific DNA fragments based on DNA complementarity and high temperature resistant DNA polymerase. Conventional PCR machines currently in use have as many as dozens of PCR reaction tanks that can be precisely controlled, which allows the PCR machine to perform PCR reactions with different reaction conditions simultaneously and in large quantities. However, it is time-consuming and expensive for quick and simple DNA testing. Therefore, microelectromechanical system-based PCR microanalysis systems are being used more and more. In the PCR micro-analysis systems disclosed in patents such as CN104745446A and CN202744555U, the PCR reaction mixture reciprocates in the micro-channel through a preset constant tem...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/686
CPCC12Q1/686C12Q2565/629
Inventor 周飞冯先文杨成鹏付瑜
Owner TDK CORPARATION
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap