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Preparation method of bifida ferment filtrate composition

A fermentation product, the technology of bifidobacteria, is used in skin care preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc. Enhances whitening and moisturizing effects

Pending Publication Date: 2020-01-24
江苏巴帝恩生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to overcome the problems of large chemical pollution, mildness, and insufficient activity in cosmetics in the prior art, a method for preparing a bifid yeast fermentation product filtrate composition is proposed, including step 1: activating the shaker-activated bifidus Bacteria are inoculated into the culture medium for fermentation; Step 2: After the fermentation is completed, inactivate the bacterial liquid, and collect the supernatant after low-speed centrifugation; Step 3: Extract the tremella at high temperature, stir and filter, and add preservatives; Step 4 : Grind the wild soybeans, after ultrasonic extraction, heating extraction and reflux stirring extraction, filter, concentrate, separate and purify, and configure it into a butanediol solution; Step 5: Take the fermentation broth in Step 2, and add different molecular weights in proportion Hyaluronic acid, niacinamide, tremella extract in step 3, wild soybean extract in step 4, heated and stirred to dissolve to obtain the final composition

Method used

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  • Preparation method of bifida ferment filtrate composition
  • Preparation method of bifida ferment filtrate composition
  • Preparation method of bifida ferment filtrate composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Inoculate the bifidobacterium activated on the shaking table into the bifidobacterium medium (BBL liquid medium) for fermentation. The fermentation conditions are: the bifidobacterium used is lactic acid (B.lactis) bifidobacterium, and the pH range 6.5~7.0, the fermentation temperature is 37°C, the shaker speed is 100rpm, and the fermentation time is 5 hours.

[0031] 2. After the fermentation is over, inactivate the bacterial liquid, use a TDL-80-2B low-speed centrifuge at a speed of 7000 rpm to centrifuge the bacterial liquid, and collect the centrifuged supernatant for later use

[0032] 3. Tremella extraction temperature is 80°C, material-to-liquid ratio is 1:4, extraction time is 8 hours, stirring extraction, stirring speed 200 rpm, sampling detection solid content is greater than 1%, that is, extraction is complete, filter while hot (Low temperature does not move), filter with 1um filter paper, add 1,2-hexanediol complex p-hydroxyacetophenone as a preservative....

Embodiment 2

[0036] 1. Inoculate the bifidobacterium activated on the shaking table into the bifidobacterium medium (BBL liquid medium) for fermentation. The fermentation conditions are: the bifidobacteria used are lactic acid (B.lactis) bifidobacteria, and the pH range 6.5~7.0, the fermentation temperature is 39°C, the shaker speed is 120rpm, and the fermentation time is 7 hours.

[0037] 2. After the fermentation is over, inactivate the bacterial liquid, use a TDL-80-2B low-speed centrifuge at a speed of 7000 rpm to centrifuge the bacterial liquid, and collect the centrifuged supernatant for later use

[0038]3. The temperature for tremella extraction is 90°C, the ratio of solid to liquid is 1:4, and the extraction time is 6 hours. Stir and extract at a stirring speed of 200 rpm. Sampling and detection of solid content is greater than 1%, that is, the extraction is complete, and filtered with gauze while it is hot (Low temperature does not move), filter with 1um filter paper, add 1,2-hex...

Embodiment 3

[0042] 1. Inoculate the bifidobacterium activated on the shaking table into the bifidobacterium medium (BBL liquid medium) for fermentation. The fermentation conditions are: the bifidobacterium used is lactic acid (B.lactis) bifidobacterium, and the pH range 6.5~7.0, the fermentation temperature is 41°C, the shaker speed is 120rpm, and the fermentation time is 8 hours.

[0043] 2. After the fermentation, inactivate the bacterial liquid, use a TDL-80-2B low-speed centrifuge at a speed of 7000 rpm to centrifuge the bacterial liquid, and collect the centrifuged supernatant for later use.

[0044] 3. Tremella extraction temperature is 95°C, material-to-liquid ratio is 1:5, extraction time is 4 hours, stirring extraction, stirring speed 200 rpm, sampling detection solid content is greater than 1%, that is, extraction is complete, filter while hot (Low temperature does not move), filter with 1um filter paper, add 1,2-hexanediol complex p-hydroxyacetophenone as a preservative.

[00...

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Abstract

The invention provides a preparation method of a bifida ferment filtrate composition, and relates to the technical field of microbial fermentation and cosmetics. The preparation method of the bifida ferment filtrate composition comprises the steps of (1) inoculating a bifidobacterium activated by a shaking table into a culture medium, and fermenting; (2) after finishing fermenting, inactivating abacteria solution, and collecting a liquid supernatant after low-speed centrifugation; (3) extracting essences from tremella fuciformis at high temperature, stirring and filtering, and adding a preservative; (4) smashing wild soybean, carrying out ultrasonic extraction, heating extraction and backflow stirring extraction, filtering, concentrating, separating, purifying, and preparing a butanediolsolution; and (5) taking fermentation liquor in the step (2), adding hyaluronic acid, nicotinamide, the tremella fuciformis extract in the step (3) and the wild soybean extract in the step (4) with different molecular weights according to the proportion, heating, stirring and dissolving to obtain the final composition. The preparation method of the bifida ferment filtrate composition provided by the invention can effectively solve the problems of high chemical pollution and insufficient tenderness and activity existed in cosmetics.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation and cosmetics, in particular to a preparation method of a bifid yeast fermentation product filtrate composition. Background technique [0002] As fermented products are well known by the majority of users, people's awareness of fermented products is also getting deeper and deeper. The filtrate of the fermented product obtained after fermentation by bifidobacteria is rich in amino acids, polypeptides, minerals and various vitamins, which are important components of the human body and play an important role in improving skin conditions. However, with the continuous increase of fermented products, the quality of fermented products has also attracted much attention. [0003] Chinese patent CN108272645A discloses an anti-aging facial mask essence containing lysate of bifid yeast fermentation product and its preparation method, which uses formula components: oat β-glucan, water-locking m...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/9789A61K8/9728A61K8/73A61K8/67A61Q19/00A61Q19/02A61Q19/08
CPCA61K8/99A61K8/9728A61K8/9789A61K8/735A61K8/675A61Q19/00A61Q19/02A61Q19/08A61K2800/85A61K2800/82
Inventor 陈秀玉李俊宏卢勇詹柱
Owner 江苏巴帝恩生物科技有限公司
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