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Anti-browning culture method of Haworthisa.spp cells

A culture method and cell culture technology, applied in the field of anti-browning culture of Yulu cells, can solve the problems of no proliferation and anti-browning, no significant difference in seed germination rate, etc., and achieve vigorous growth and division ability and fast growth rate. Effect

Pending Publication Date: 2020-01-24
JALA GROUP CORPORATION
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Scholars have studied the use of Qilian Mountain glacier water, pure water and distilled water to germinate plant seeds and plant tissue culture, and found that there is no significant difference in seed germination rate, and only small pieces of green callus were induced in tissue culture. However, it has not been found that the glacier water can promote the proliferation of plant cell tissue and prevent browning

Method used

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  • Anti-browning culture method of Haworthisa.spp cells
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  • Anti-browning culture method of Haworthisa.spp cells

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Embodiment 1

[0040] (1) First, under sterile conditions, quantitatively unbrown Yulu ( Haworthisa . spp) cell culture was inoculated into a 150ml sterile Erlenmeyer flask, and 40ml of sterile culture medium was added. The experiment was divided into two groups. One group of culture liquid used glacier water as solvent, and the other group of culture liquid used ultrapure water as solvent. The formula of culture liquid was MS +6-BA1.0 mg / L+NAA0.1 mg / L+ Sucrose 30 g / L, the initial pH value is 5.8±0.1 (the fluctuation does not exceed 0.1). Then place the flask on a shaking table with a temperature of 25°C and a rotation speed of 100 rpm / min for cell suspension culture. After 11 days, the cells were harvested, the state of the cells was observed, the wet weight of the cells was weighed, and the experimental data were recorded. See Table 1 for details.

[0041] Among them, multiplication factor = final cell wet weight / initial cell wet weight

[0042] see figure 2 , Table 1. Yulu cells cult...

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Abstract

The invention relates to an anti-browning culture method of Haworthisa.spp cells. The method is characterized in that the method comprises the following steps: 1, inoculating the Haworthisa.spp cell culture into a sterile triangular flask, adding a culture solution into the triangular flask, putting the sterile triangular flask on a shaking table for proliferation suspension culture, taking the filtrate of the Haworthisa.spp cell culture obtained in the previous step, adding the culture solution, inoculating the material into the sterile triangular flask, and putting the sterile triangular flask on the shaking table for anti-browning suspension culture. The method directly takes the callus cells of the Haworthisa.spp as explants, firstly carries out suspension culture and then carries outsolid culture; meanwhile, the Himalaya glacier water is used for culturing the Haworthisa.spp cell tissue, and the glacier water can be used as an anti-browning agent, so that the browning phenomenonin the Haworthisa.spp cell tissue culture can be effectively prevented and solved, and can also be used as an additive to be added into a culture medium to promote proliferation of plant cell tissues,and scientific reference is provided for tissue culture and industrialization of the Haworthisa.spp cells.

Description

technical field [0001] The invention relates to the field of plant cultivation, in particular to an anti-browning cultivation method of Yulu cells. Background technique [0002] Yulu is a succulent plant. Its succulent leaves are transparent like jade, crystal and round, and can be placed on the balcony or desk like a work of art. Therefore, it is very popular among succulent plant lovers. There are many varieties of Yulu gardening, and it is sought after by people because of its crystal clear and varied leaf color, which leads to a hot market and high prices. Its propagation and cultivation methods can include division, cutting, leaf cutting, sowing and so on. [0003] In the process of plant tissue culture, browning is a common phenomenon and will directly affect the success of tissue culture. This phenomenon is that the explants are browned and browned due to the influence of phenolic compounds secreted by themselves. In the process of plant tissue culture, such as call...

Claims

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Application Information

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IPC IPC(8): C12N5/04C12N5/02
CPCC12N5/04C12N5/0025
Inventor 李惠玲章漳
Owner JALA GROUP CORPORATION
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