Method for preparing immobilized enzyme from zeolite imidazate skeleton compound

Abstract

The invention discloses a method for preparing immobilized enzyme from a zeolite imidazate skeleton compound. The method comprises the following steps: respectively preparing a 2-methylimidazole solution, a zinc acetate aqueous solution and a polyethylene glycol aqueous solution; centrifuging an enzyme stock solution, removing a supernate, adding the polyethylene glycol aqueous solution, carryingout ultrasonic treatment to obtain a polyethylene glycol / carboxypeptidase A compound solution, carrying out ultrafiltration on the compound solution, and transferring the polyethylene glycol / carboxypeptidase A compound into the 2-methylimidazole solution by using the 2-methylimidazole solution to form a mixed solution; and adding the zinc acetate aqueous solution into the mixed solution, magnetically stirring, performing steps of standing and aging, centrifugally collecting a solid product, washing with water, and drying the material to obtain the immobilized enzyme prepared from the zeolite imidazate skeleton compound. According to the method, the stability and the loading rate of the enzyme can be greatly improved, the storage time of the enzyme is prolonged, and the prepared zeolite imidazate skeleton compound-loaded carboxypeptidase A compound is simple in recovery operation, economical, efficient and high in reusability.

Description

technical field

[0001] The invention relates to immobilized enzyme technology, which is suitable for industrial fields such as grain food safety, and in particular relates to a method for preparing immobilized enzyme from a zeolite imidazolate skeleton compound. Background technique

[0002] Enzyme immobilization methods include physical adsorption, coupling, interception, embedding, and cross-linking. Metal-organic framework compounds can be used as carrier materials for enzyme immobilization. Adsorb enzymes into the pores of pre-synthesized metal-organic framework compounds. Although this immobilization of enzymes can be achieved through simple steps, since the pore size of most metal-organic The method of forming complex enzymes has certain limitations. Even if the metal-organic framework compound has a pore size similar to or larger than that of the enzyme, if the binding between them is relatively weak, the enzyme is likely to be washed away, and the existing adsorptio...

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