A kind of hormone combination and application thereof for enhancing ACS enzyme activity

An enzyme activity and hormone technology, applied in the field of plant molecular biotechnology and genetic engineering, can solve the problems of unable to promote fruit softening and edible state, unable to effectively promote fruit ethylene release, etc., to promote ethylene release, accelerate post-ripening process, promote The effect of elevated ACC content

Active Publication Date: 2021-02-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when methyl jasmonate was used to treat postharvest kiwi fruit, it could not effectively promote the release of ethylene in the fruit, nor could it impel the fruit to reach a softened and edible state (Example 2)

Method used

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  • A kind of hormone combination and application thereof for enhancing ACS enzyme activity
  • A kind of hormone combination and application thereof for enhancing ACS enzyme activity
  • A kind of hormone combination and application thereof for enhancing ACS enzyme activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The 'Hayward' kiwi fruit was harvested in a commercial orchard in Shaanxi Province in 2018 and reached commercial maturity. Choose fruits with the same fruit shape and size without obvious mechanical damage. At 20°C, the fruit was directly sealed in a 20L barrel for 24 hours, then taken out and stored at 20°C, and samples were taken every two days to determine relevant indicators. In this embodiment, the fruit is used as a control.

Embodiment 2

[0018] Compared with Example 1, the optimal measure is to seal the fruit in a 20L barrel at 20°C, treat it with 100 μM / L methyl jasmonate for 12 hours, then open the lid of the barrel and store it in normal air for 12 hours. Finally, it was stored at 20°C, and samples were taken every two days to determine relevant indicators.

Embodiment 3

[0020] Compared with Example 1, the optimal measure is to seal the fruit in a 20L bucket at 20°C, treat it with 100μl / L ethylene for 12 hours, then open the lid of the bucket and store it in normal air for 12 hours. Finally, it was stored at 20°C, and samples were taken every two days to determine relevant indicators.

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Abstract

The invention provides a hormone combination for enhancing ACS enzyme activity and application thereof, which consists of 100 μl / L of ethylene and 100 μM / L of methyl jasmonate. The expression levels of AdNAC2 and AdNAC3 in kiwi fruit at the commercial maturity stage were significantly increased after the hormone combination treatment, and could activate the 1-aminocyclopropane-1-carboxylic acid (ACC) synthetase coding in the ethylene biosynthetic pathway The promoter activity of the gene AdACS1 can significantly enhance the ACS enzyme activity and promote the increase of ACC content; the ethylene release in the treated fruit can be advanced 6 days, the hardness and soluble solid content can reach the edible state 4 days in advance, and the post-ripening process is significantly improved. accelerate. The hormone combination of the invention can enhance the expression of the transcription factor NAC gene, thereby increasing the activity of the ACS enzyme in the ethylene metabolism pathway, promoting the release of ethylene from the fruit, and accelerating the post-ripening process of the fruit.

Description

technical field [0001] The invention belongs to the fields of plant molecular biotechnology and genetic engineering, relates to a hormone combination for enhancing ACS enzyme activity, and is a novel ethylene-methyl jasmonate hormone combination treatment for enhancing ACS enzyme activity in ethylene metabolism pathway and its application. Background technique [0002] Kiwi fruit, because it is rich in more than 10 kinds of amino acids such as leucine, phenylalanine and tyrosine, as well as various vitamins, balanced mineral elements, dietary fiber and other metabolite nutrients that are beneficial to human health, is known as "King of Fruits" and "King of Vc". Kiwi fruit originated in China, and it was domesticated and cultivated in New Zealand around the beginning of the 20th century. After decades of efforts to domesticate and select wild kiwi fruit, many varieties with novel consumption characteristics have been developed. It has been planted on a large scale in three c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N27/00A01N37/42A01P21/00A23B7/154A23B7/04
CPCA01N27/00A01N37/42A23B7/04A23B7/154A23V2002/00A23V2200/10
Inventor 殷学仁吴莹莹刘晓芬王戬傅蓓凌王文球童扬
Owner ZHEJIANG UNIV
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