Medicinal and edible homologous composition for preventing and treating chronic alcoholic liver disease and application
A technology of alcoholic liver disease, medicine and food homology, applied in the field of medicine, can solve the problems of slow curative effect of traditional Chinese medicine, excessive dosage, toxic and side effects, etc., achieve lower MDA level, strong preventive effect, reduce cell fatty degeneration and inflammatory infiltration Effect
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Embodiment 1
[0040] Example 1 Chinese Medicine Composition for Preventing Chronic Alcoholic Liver Disease
[0041] Preparation of pharmaceutical composition
[0042] (1) Preparation of Salvia miltiorrhiza extract (DS): Clean 500g of Danshen decoction pieces, add 12 times the amount of distilled water, cook for 3.0 hours, and filter; add 10 times the amount of distilled water to the residue, continue cooking for 3.0 hours, and filter; then The two filtrates were heated at 60°C for 8 hours, and then filtered with double-layer filter paper, and the filtrate was placed in a rotary evaporator to obtain a concentrated solution; finally, the obtained concentrated solution was centrifuged in a centrifuge at a speed of 8000r / min for 20 minutes, and the supernatant was removed. Danshen extract.
[0043] (2) Preparation of Schisandra chinensis extract (WWZ): Clean 500g of Schisandra chinensis decoction pieces, add 12 times the amount of distilled water, cook for 3.0h, filter; add 10 times the amount...
Embodiment 2
[0046] Example 2 Construction of Animal Model of Chronic Alcoholic Liver Disease
[0047] C57 mice (4-week-old, female) were first given a liquid glucose diet (Bio Serv, USA) for 3 days to adapt to the liquid diet. Subsequently, mice in the model group will be fed a liquid ethanol diet (Bio Serv, USA) for 14 days. The control group continued to receive a liquid glucose diet (Bio Serv, USA) for 14 days. On the 15th day of feeding liquid ethanol, the mice in the model group were given 5 g / kg ethanol by intragastric administration once.
Embodiment 3
[0048] The effect of embodiment 3 Chinese medicine composition on animal model
[0049] 1. Experimental method
[0050] (1) Experimental grouping
[0051] Take the Chinese medicine composition prepared in Example 1 as an example for research.
[0052] The mice with chronic alcoholic liver disease were divided into a model control group and a Chinese medicine treatment group. The Chinese medicine treatment group included Danshen group (DS), Schisandra chinensis group (WWZ), Jiaogulan group (JGL), and liver-protecting Chinese medicine mixture group (LF). Each treatment group is divided into high-dose group (H), middle-dose group (M) and low-dose group (L). Through intragastric administration, the model control group was given the same dose of normal saline.
[0053] (2) Detection of relevant indicators
[0054] On the 16th day after feeding liquid ethanol, the mice were sacrificed by cervical dislocation, blood was collected from the heart, and the blood and liver homogenate...
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