A kind of method utilizing compound enzyme to prepare maltooligosaccharide syrup
A technology of oligosaccharide syrup and compound enzyme, applied in the field of malto-oligosaccharide production, can solve problems such as high cost, achieve low cost, avoid the influence of production and processing, and achieve the effects of high content
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0024] Example 1: Simultaneous addition of cyclodextrin glucosyltransferase and cyclodextrin hydrolase
[0025] Dissolve corn starch in a pH 6 buffer system to prepare 5% starch milk, add 7.8U / g dry base starch cyclodextrin glucosyltransferase and 21.6U / g dry base starch cyclodextrin after gelatinization Hydrolase, react at 70°C for 24h, take out part of the reaction samples at 1h, 6h, 12h, and 24h respectively, and stop the reaction in a boiling water bath to obtain maltooligosaccharide syrup. The content of maltooligosaccharides was measured by high performance liquid phase, and the results were as follows: figure 1 and figure 2 shown. During the compound enzyme reaction, the substrate conversion rate can reach 61.0%, and the ratio of maltose to maltooctaose is: maltose: 16.4%; maltotriose: 16.9%; maltotetraose: 31.6%; maltopentaose: 12.7% ; Maltohexaose: 6.5%; Maltoheptaose: 6.9%; Maltooctaose: 9.0%, showing a good preparation effect of maltooligosaccharides, in which t...
Embodiment 2
[0026] Example 2: Simultaneous addition of cyclodextrin glucosyltransferase and cyclodextrin hydrolase
[0027] Dissolve corn starch in a pH 6 buffer system to prepare 5% starch milk, add 7.8U / g dry base starch cyclodextrin glucosyltransferase and 21.6U / g dry base starch cyclodextrin after gelatinization Hydrolase, reacted at 70°C for 24h, and terminated the reaction in a boiling water bath. The content of reducing sugar and glucose in the obtained maltooligosaccharide syrup was measured by DNS method and glucose assay kit respectively. The results were as follows: image 3 with Figure 4 . Depend on image 3 with Figure 4 It can be seen that the utilization rate of the substrate is improved during the action of the compound enzyme, and the effect of the compound enzyme on improving the substrate utilization rate and promoting the reaction efficiency is obvious, and by Figure 4 It can be seen that the compound enzyme does not increase the content of glucose, and the infl...
Embodiment 3
[0029] Dissolve tapioca starch in a pH 5.5 buffer system to form 10% starch milk, add 10.6U / g dry base starch cyclodextrin glucosyltransferase and 42.6U / g dry base starch cyclodextrin after gelatinization The hydrolytic enzyme was reacted at 70° C. for 24 hours, and the reaction was terminated in a boiling water bath to obtain maltooligosaccharide syrup. The proportion of maltooligosaccharides in the syrup with a polymerization degree of four and above accounted for 71.3%, and the substrate conversion rate reached 65.8%.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More