Fucosidase and application of fucosidase to preparation of Bombay phenotype red blood cells

Abstract

The invention belongs to the field of glycobiology technology and engineering, and particularly relates to novel fucosidase and enzymatic activity and application thereof. The fucosidase can effectively act on polysaccharide structures located on the cell surfaces, and the fucosidase is named as cell surface fucosidase I (csFase I). It is found and proved that csFase I can effectively remove alpha-1,2 fucose on a H-antigen on the cell surfaces of active red blood cells, can effectively convert O-type red blood cells into rare Bombay phenotype red blood cells, and can provide a simple and fastnew way for solving the current situation of extremely difficult blood supply to patients with a Bombay phenotype blood type; and a new method is provided for the research of glycobiology and biomedicine.

Description

technical field [0001] The invention belongs to the field of glycobiology technology and engineering, and relates to the preparation method and application of fucosidase, especially the application in the preparation of Bombay-type red blood cells. Background technique [0002] The prior art discloses that glycosylation modification is a major modification on the cell surface, and plays an extremely important function in the recognition process between cells; as a kind of glycosylation, fucosylation widely exists in various The cell surface also plays an important biological function [1]. [0003] L-fucose is widely found in mammalian, plant and insect cells, and glycoconjugates containing L-fucose play a vital role in many physiological and pathological processes of these organisms, such as inflammatory response, Bacterial and viral infections, tumor metastasis, occurrence of genetic diseases, fertilization process, immune activation effects of antibodies, and allergic rea...

AI Technical Summary

Problems solved by technology

Since most people's blood types are A, B, AB or O, and the population distribution of each blood type is not very different, when a patient urgently needs blood products, each blood transfusion institution will have a certain amount of support for the four blood types. Inventory to ensure the normal supply of blood, even in the case of emergency blood supply, it is relatively easy to find a suitable blood donor, but for patients with Bombay blood type or part of Bombay blood type, the situation is not optimistic, in the case of urgent need for blood products In particular, it is often difficult to find a suitable donor when the demand for red blood cells is high. In addition, in some cases, the human body will produce a relatively high level of autoantibodies to induce autoimmune diseases. Studies have found that using glycan When autoantibodies in 106 experimenters were detected by the chip, it was found that 4 of them had autoantibodies against the type 2 H structure in their serum[19]. Type erythrocytes are also the best solution, so converting type O erythrocytes to Bombay type erythrocytes lacking H antigen will effectively solve the above problems

[0007] Type O blood is generally considered to be "universal blood" because O-type red blood cells can be transfused to individuals of any blood type A, B, and AB. Type individuals, and this point is often overlooked because of Bombay and Bombay-like blood rareness; Bombay-type or Bombay-like individuals can only receive H-antigen-deficient red blood cells, and clinically H-deficient Bombay-type red blood cell donors are extremely rare. There is currently no effective method for converting type O red blood cells to Bombay type red blood cells

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