Process for extracting barley beta-amylase

Abstract

The invention belongs to the technical field of bio-enzyme preparations and in particular discloses a process for extracting barley beta-amylase. A filter pressing device used in the process comprisestwo beams, wherein a hydraulic cylinder is fixed at one end of the beams; a pressing plate is fixed at the output shaft end of the hydraulic cylinder; the hydraulic cylinder is used for driving a pressing plate to move; a baffle plate is fixed at the other end far away from the hydraulic cylinder, of the beams; two or more filtering frames are moveably arranged between the two beams; and water tubes are arranged at top ends of both the pressing plate and the baffle plate. Due to arrangement of an extrusion airbag, filter residues can be extruded through the extrusion airbag, then moisture inthe filter residues can be extruded out, the utilization rate of raw material slurry can be increased, furthermore, the filter pressing efficiency of the filter pressing device is improved, an arranged connecting rod is rotated under extrusion of the extrusion airbag, when air is released from the extrusion airbag, a filter cake can be easily dissociated from a filtering frame because of vibrationcaused by returning of the connecting rod, and thus the filter pressing efficiency of the filter pressing device can be improved.

Description

technical field [0001] The invention belongs to the technical field of biological enzyme preparations, in particular to a barley β-amylase extraction process. Background technique [0002] β-amylase (β-amylase), also known as starch β-1,4-maltosidase, is a kind of amylase, which is widely found in higher plants such as barley, wheat, sweet potato, soybean, and Bacillus, etc. in microorganisms. It is the main saccharification agent for beer brewing and caramel (malt syrup) production. Using β-amylase produced by microorganisms such as Bacillus polymyxa and Bacillus megaterium to saccharify starch raw materials that have been acidified or liquefied by α-amylase can produce high maltose syrup with a maltose content of 60% to 70%. [0003] When barley β-amylase obtains the concentrated enzyme solution, it needs to use the pressure filtration step, but the traditional steps are cumbersome, time-consuming and labor-intensive, and the filter cake during the pressure filtration pr...

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Problems solved by technology

[0003] When barley β-amylase obtains the concentrated enzyme solution, it needs to use the pressure filtration step, but the traditional steps are cumbersome, time-consuming and labor-intensive, and the filter cake during the pressure filtration process contains moisture, resulting in the loss of the pressure filtration solution. In addition, The filter cake is not easily separated from the filter press device, resulting in low filter press efficiency of the enzyme liquid, which affects the extraction of barley β-amylase. Accordingly, the present invention proposes a barley β-amylase extraction process

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