Streptomyces albus for preventing and controlling aspergillus flavus and aflatoxin and application thereof

A technology of streptomyces albicans and aflatoxin, applied in the direction of application, chemicals for biological control, bacteria, etc., can solve the problems of no biological control bacteria, etc., achieve the effect of inhibiting pollution and wide application prospects

Active Publication Date: 2020-02-11
MINJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Searched for invention patent applications related to aflatoxin control at home and abroad, but no invention patent application for biocontrol bacteria from the intestinal tract of marine animals

Method used

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  • Streptomyces albus for preventing and controlling aspergillus flavus and aflatoxin and application thereof
  • Streptomyces albus for preventing and controlling aspergillus flavus and aflatoxin and application thereof
  • Streptomyces albus for preventing and controlling aspergillus flavus and aflatoxin and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 strain screening

[0028] Purchase large and healthy sea cucumbers from the aquatic market, use 75v / v% alcohol to disinfect the surface of the sea cucumbers, cut open the abdomen with a scalpel, take out the intestinal contents, immediately place them in 20ml sterile water, and vortex , to obtain a homogeneous suspension. According to 100ul of each plate, evenly spread on the seawater oligonutrient screening medium, place it at 28°C for static culture for 10 days, and pick a single colony for antibacterial experiment.

Embodiment 2

[0029] Embodiment 2 Streptomyces albicans HSB5 antibacterial experiment

[0030] The antibacterial experiment was carried out on the PDA plate, using Streptomyces albicans HSB5 in bold and the clinical drug Amphotericin to carry out the experiment of resisting pathogenic fungus Aspergillus flavus. Set 3 concentration gradients: 1ug / ml, 5ug / ml, 10ug / ml, the leftmost one is the blank control. The results of the experiment found that both Streptomyces albicans HSB5 and amphotericin significantly inhibited the growth and sporulation of Aspergillus flavus, and the antibacterial activities of the two were equivalent, and their MICs were both 1ug / ml ( figure 1 ). figure 2 It is the growth figure of Streptomyces on the plate and the inhibitory effect of Streptomyces HSB5 on the growth of Aspergillus flavus, by figure 2 It can be seen that Streptomyces cyanogenes HSB5 can significantly inhibit the growth and sporulation of Aspergillus flavus.

Embodiment 3

[0031] Embodiment 3 suppresses the experiment of Aspergillus flavus producing toxin

[0032]Streptomyces albicans HSB5 was fermented with MTSB medium, the fermentation conditions were: temperature 28°C, rotation speed 150 rpm, and fermentation days 10 days. After the fermentation, the bacteria and the fermentation broth were separated by centrifugation, and an equal volume of ethyl acetate was added to extract twice to obtain a crude substance.

[0033] Five groups were set up in the toxin production experiment, inoculated with Aspergillus flavus spores (final concentration 10 5 / ml) in YES liquid medium, then add 0, 1 ug / ml, 5 ug / ml, 10 ug / ml, 20 ug / ml of Streptomyces albicans in bold, and culture at 28°C for 6 day, collect the culture. Add an equal volume of dichloromethane to extract aflatoxin, and use thin layer chromatography to determine the content of aflatoxin in different groups. The experimental results found that when the concentration of HSB5 bold substance adde...

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Abstract

The invention relates to a strain of streptomyces albus for preventing and controlling aspergillus flavus and aflatoxin and application thereof. Microbes are separated from intestinal tracts of sea cucumbers under an oligotrophic culture condition by using a culture medium only containing agar powder; and in addition, the strain of streptomyces albus is separated from the intestinal tracts of healthy sea cucumbers. After being fermented, the streptomyces albus generates a great number of active secondary metabolites capable of inhibiting the growth of a pathogenic fungus of the aspergillus flavus and the synthesis of the aflatoxin. In addition, the streptomyces albus can completely inhibit the pollution of fungi and fungal toxin in the storage process of grains such as peanuts; and wide application prospects are realized in the field of grain safety.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to the field of control of harmful microorganisms. A strain of Streptomyces albicans HSB5 is isolated from the intestinal tract of sea cucumbers by using seawater culture medium. Streptomyces HSB5 and its extracts were used to inhibit the growth of the pathogenic fungus Aspergillus flavus and the pollution caused by aflatoxin, and the active components in the crude extract were identified by LC-MS technology. Background technique [0002] Aflatoxins are secondary metabolites produced by fungi such as Aspergillus flavus and Aspergillus parasiticus, and are a class of difuroxinone compounds. According to the difference in chemical structure, aflatoxins are mainly divided into 6 subtypes: B1, B2, G1, G2, M1 and M2, among which B1 is the most toxic. So far, aflatoxin B1 (Aflatoxin, AFB1) is recognized as the most carcinogenic natural poison in the world, and is recognized a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23B9/28A01N63/34A01P3/00C12R1/47
CPCA23B9/28A23V2002/00C12N1/205C12R2001/47A23V2200/10
Inventor 姚光山陈建明王宗华
Owner MINJIANG UNIV
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