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Artificial ligament having immunization bone formation function and preparation method of artificial ligament

An artificial ligament and functional technology, applied in the medical field, can solve problems such as low biomechanical properties

Inactive Publication Date: 2020-02-14
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Currently, the existing PET artificial ligaments heal with the host bone to form fibrous scars due to immune rejection in the host body, resulting in low biomechanical properties
In the prior art, there is no modification technology specifically targeting the immune function of artificial ligaments. Therefore, it is very important to promote the integration of the graft-bone interface and improve the biomechanical properties through immune regulation.

Method used

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  • Artificial ligament having immunization bone formation function and preparation method of artificial ligament
  • Artificial ligament having immunization bone formation function and preparation method of artificial ligament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Such as figure 1 Shown, a kind of artificial ligament preparation method with immune osteogenesis function, comprises steps:

[0026] (1) Put the PET artificial ligament of certain size in the ultrasonic machine, carry out ultrasonic cleaning to this PET artificial ligament with 75% ethanol, the time is 25min; Then use deionized water to clean described PET artificial ligament; Finally put The PET artificial ligament was naturally dried at room temperature for 24 hours;

[0027] (2) Cut the PET artificial ligament obtained in step (1), put the cut PET artificial ligament into a 100mL beaker, then add 25mL of Tris-HCL solution with a pH of 8.5 into the beaker, and stir with a small rotor Mix the PET artificial ligament with the Tris-HCL solution fully; then add 25 mL of dopamine hydrochloride solution with a concentration of 4 mg / mL into the beaker, and react for 1 hour; then take out the PET artificial ligament and wash off the excess surface of the PET artificial liga...

Embodiment 2

[0031] Such as figure 1 Shown, a kind of artificial ligament preparation method with immune osteogenesis function, comprises steps:

[0032] (1) Put the PET artificial ligament of certain size in the ultrasonic machine, carry out ultrasonic cleaning to this PET artificial ligament with 75% ethanol, the time is 25min; Then use deionized water to clean described PET artificial ligament; Finally put The PET artificial ligament was naturally dried at room temperature for 24 hours;

[0033] (2) Cut the PET artificial ligament obtained in step (1), put the cut PET artificial ligament into a 100mL beaker, then add 25mL of Tris-HCL solution with a pH of 8.7 into the beaker, and stir with a small rotor Mix the PET artificial ligament with the Tris-HCL solution fully; then add 25 mL of dopamine hydrochloride solution with a concentration of 4 mg / mL into the beaker, and react for 12 hours; then take out the PET artificial ligament and wash off the excess surface of the PET artificial li...

Embodiment 3

[0037] Such as figure 1 Shown, a kind of artificial ligament preparation method with immune osteogenesis function, comprises steps:

[0038] (1) Put the PET artificial ligament of certain size in the ultrasonic machine, carry out ultrasonic cleaning to this PET artificial ligament with 75% ethanol, the time is 25min; Then use deionized water to clean described PET artificial ligament; Finally put The PET artificial ligament was naturally dried at room temperature for 24 hours;

[0039] (2) Cut the PET artificial ligament obtained in step (1), put the cut PET artificial ligament into a 100mL beaker, then add 25mL of Tris-HCL solution with a pH of 9.0 into the beaker, and stir with a small rotor Mix the PET artificial ligament with the Tris-HCL solution fully; then add 25 mL of dopamine hydrochloride solution with a concentration of 4 mg / mL into the beaker, and react for 24 hours; then take out the PET artificial ligament and wash off the excess surface of the PET artificial li...

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Abstract

The invention discloses an artificial ligament having immunization bone formation function and a preparation method of the artificial ligament. The preparation method comprises the steps of (1) placing a PET artificial ligament in an ultrasonic machine, performing cleaning, and performing drying by airing; (2) cutting out the PET artificial ligament obtained in the step (1), placing the PET artificial ligament which is cut out in a beaker loaded with a Tris-HCL solution, adding 25mL of a dopamine hydrochloride solution to the beaker, and performing a reaction for 1-24h, to obtain a PET ligament modified by PDA; (3) placing the PET ligament modified by the PDA, obtained in the step (2) in a 50mL centrifugal tube, and then adding a CS-ADH solution to obtain a PET ligament modified by CS-PDA;and (4) slowly and uniformly dropwise adding 1-20ug of an rhBMP-2 solution to the surface of the PET ligament obtained in the step (3), performing freezing at minus 20 DEG C, performing staying overnight, and performing freeze drying in a bacteria-free freeze dryer so as to obtain the artificial ligament. The dopamine adopted for the artificial ligament is adsorbed to the PET through an autopolymerization reaction, and then through a reaction of the dopamine and chondroitin sulfate-ADH, chondroitin sulfate is connected to the surface of PET fibers, so that an immunoregulation function is provided for the PET artificial ligament.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to an artificial ligament with immune osteogenesis function and a preparation method thereof. Background technique [0002] Currently, the existing PET artificial ligaments heal with the host bone to form fibrous scars due to immune rejection in the host body, resulting in low biomechanical properties. In the prior art, there is no modification technology specifically targeting the immune function of artificial ligaments. Therefore, it is very important to promote the integration of the graft-bone interface and improve the biomechanical properties through immune regulation. Contents of the invention [0003] In order to solve the above-mentioned problems in the prior art, the present invention proposes an artificial ligament with immune osteogenesis function and a preparation method thereof. [0004] To achieve the above object, the present invention adopts the following technic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/20A61L27/22A61L27/54
CPCA61L27/18A61L27/20A61L27/227A61L27/54A61L2300/252A61L2300/412A61L2300/426A61L2400/18A61L2430/10C08L5/08C08L67/02C08L79/02C08L89/00
Inventor 刘昌胜赵金忠何宏燕蒋佳李亚民吴敬尧
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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