Lactobacillus plantarum zjuf T17 and its application

A technology of Lactobacillus plantarum and drugs, applied in the directions of Lactobacillus, application, bacteria, etc., can solve the problem of slightly lower fermentation capacity, achieve high survival rate, reduce cholesterol content, and alleviate the effects of weight gain

Active Publication Date: 2020-08-04
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation of fermented milk by Lactobacillus plantarum has also been reported, but its fermentability is still slightly lower than that of Lactobacillus casei Shirota et al. [Angmo, K., et al., Probiotic characterization of lactic acid bacteria isolated from fermented foods and beverage of Ladakh. Technology,2016.66:p.428-435.], the ability to develop fermented milk of Lactobacillus plantarum has certain prospects

Method used

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  • Lactobacillus plantarum zjuf T17 and its application
  • Lactobacillus plantarum zjuf T17 and its application
  • Lactobacillus plantarum zjuf T17 and its application

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Embodiment 1

[0033] 1. Screening and isolation of strains

[0034] Weigh 5g of sourdough and add it to 45mL 0.85% sterilized saline solution, vibrate to make a suspension, carry out serial gradient dilution, take 10% after dilution -5 、10 -6 、10 -7 Three gradients were spread on the MRS medium, and each gradient was made in triplicate. Put the coated plate into an anaerobic incubator at 37°C for 24-48h. The colonies with obvious differences were selected and separated by streaking on the MRS medium, and the plate was placed in an anaerobic incubator at 37°C for 24-48h. After streaking for 3-5 times in a row, take a drop of 5% hydrogen peroxide that is now prepared and drop it on the glass slide, pick a single colony on the solid medium and insert it into the 5% hydrogen peroxide drop and mix evenly to produce Air bubbles are positive for catalase test, no air bubbles are negative for catalase test, and strains with negative catalase test are suspected probiotic strains.

[0035] The s...

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Abstract

The invention discloses a plant lactobacillus (Lactobacillus plantarum) ZJUF T17 and an application thereof, belonging to the field of biotechnology. Lactobacillus plantarum ZJUF T17 is isolated from sourdough, and the preservation number is CCTCC NO:M 2017342. The Lactobacillus plantarum ZJUF T17 provided by the present invention has a very high survival rate and strong gastrointestinal adhesion in the gastrointestinal tract, and can significantly inhibit the reproduction of gastric and intestinal pathogenic bacteria; the Lactobacillus plantarum can significantly reduce the Medium cholesterol content, optimizing the ratio of high-density lipoprotein cholesterol to low-density lipoprotein cholesterol; the Lactobacillus plantarum can significantly reduce the TNF-α content in serum, and has the function of immune regulation; The Lactobacillus plantarum has the ability to relieve weight gain ; In addition, the plantarum lactobacillus has the potential to prepare fermented milk.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a new strain ZJUF T17 of Lactobacillus plantarum and its application. Background technique [0002] Traditional fermented foods are rich in lactic acid bacteria, which co-exist in the digestive system of the human body and play a role in preparing important nutrients by decomposing fiber and complex proteins. The microorganisms that survive in the gastrointestinal organs of animals including humans and improve the intestinal microbial environment of the host, thereby benefiting the health of the host are collectively referred to as probiotics. In recent years, with the increase of people's health awareness, the development of probiotic research has been promoted. It has been proved that probiotics can regulate gastrointestinal flora, immunity, anti-oxidation, lower cholesterol, etc., and the food produced by the fermentation of probiotics also has similar functions [Gerritsen, J., e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135A23C9/123A61K35/747A61P3/04A61P3/10C12R1/25
CPCA61P3/04A61P3/10A23C9/123A23L33/135A23V2002/00A61K2035/115C12R2001/25C12N1/205A23V2400/169A23V2200/328A23V2200/332
Inventor 冯凤琴李杨李阳孔思杰赵敏洁钟浩
Owner ZHEJIANG UNIV
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