Extracting and purification method of carminic acid in soft sweets

A technology of carminic acid and purification method, applied in the fields of food and health care products, can solve the problems of time-consuming analysis and determination, low carminic acid content, interference, etc., and achieve the effect of accurate detection

Inactive Publication Date: 2020-02-14
BY HEALTH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the detection process of spectrophotometry, what is sought is the total absorbance at a single wavelength. It cannot be ruled out that non-carminic acid also absorbs at this wavelength, which will affect the accuracy of the measurement results, and the analysis and determination are also time-consuming.
However, high performance liquid chromatography is easily interfered by the complex matrix in food, and its application is limited.
The current high-performance liquid chromatography is only a simple treatment of the sample. In the actual detection, it is difficult to effectively separate carminic acid from samples with complex matrices such as carbohydrates.
At the same time, the content of carminic acid in soft sugar samples is very low. If only simple treatment is used, carminic acid cannot be effectively detected and it is difficult to achieve the purpose of control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Extraction and Purification of Carminic Acid in Acerola Vitamin C Gummies

[0023] (1) Add 3g of acerola vitamin C soft candy sample to 20mL of water, heat in a water bath at 70°C to dissolve the sample, add 4mL of saturated sodium sulfate solution and 2mL of 1% citric acid solution in sequence, and mix well to obtain a sample solution ;

[0024] (2) Centrifuge the sample solution at 8000r / min for 5min, and add the centrifugate into a chromatography column filled with polyamide (weigh 0.5g of polyamide powder, disperse with a small amount of 1% citric acid solution, and transfer to the chromatography column) , use 10ml washing solution to elute the chromatography column, and then wash the chromatography column with water until the eluent is neutral, discard the eluent; the washing solution is 4mL of saturated sodium sulfate solution and 1mL of 1% citric acid solution , add water to 25mL of mixed aqueous solution;

[0025] (3) Then use 9mL of analytical solu...

Embodiment 2

[0026] Example 2: Extraction and Purification of Carminic Acid in Acerola Vitamin C Gummies

[0027] (1) Add 3 g of acerola vitamin C soft candy sample to 10 mL of water, heat in a water bath at 70°C to dissolve the sample, add 1 mL of saturated sodium sulfate solution and 0.5 mL of 1% formic acid solution in sequence, and mix well to obtain a sample solution ;

[0028] (2) Add absolute ethanol to the sample solution to 50mL, mix quickly, and centrifuge at 8000r / min for 5min to obtain the centrifugate and residue; wash the residue twice with 70% ethanol, 5 mL each time, combine the centrifugate and Concentrate the ethanol washing solution to about 5mL under reduced pressure at 60~80°C to obtain a concentrated solution; use 15mL of 1% citric acid solution to transfer the concentrated solution to a chromatographic column filled with polyamide (weigh 0.5 mL of polyamide powder g, disperse with a small amount of 1% citric acid solution, transfer to a chromatography column), first...

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Abstract

The invention discloses an extracting and purification method of carminic acid in soft sweets. The extracting and purification method comprises the steps: (1) water is added into samples, heating is conducted under the condition of 70-80 DEG C to enable the samples to be dissolved, a saturated sodium sulfate solution and a dilute acid solution are added in sequence, a mixture is evenly mixed, anda sample solution is obtained; (2) the sample solution is centrifuged, and centrifugate is added into a chromatographic column loaded with polyamide; (3) the chromatographic column is eluted with washing liquid, then the chromatographic column is eluted with water till eluent is neutral, and then the eluent is discarded; and (4) then the chromatographic column is eluted with analysis liquid for multiple times, the eluent is collected, a phosphoric acid solution is added, and then the carminic acid is obtained. The extracting and purification method aims at soft sweet products, the carminic acid is subjected to adsorption impurity removal through polyamide powder under an acid environment, after eluting with the analysis liquid, complex matrixes such as sugars can be effectively separated from the carminic acid, a high-purity carminic acid extracting solution is obtained, the problem of inaccurate detection caused by low content of the carminic acid in the soft sweets is solved, and thus the content of the carminic acid in the soft sweets is accurately detected.

Description

technical field [0001] The invention belongs to the technical field of health products and food, and in particular relates to a method for extracting and purifying carminic acid in soft candies. Background technique [0002] Cochineal is a natural pigment extracted from cochineal insects. It is pink to purple. It is an anthraquinone natural pigment. Unlike other natural pigments, its physical and chemical properties are very stable, and it is regarded as the safest natural pigment. Pigment, can be used as coloring agent for beverages such as wine, fruit pulp, cold drinks, candies, cakes, meat, sausage, etc. "GB 2760-2014 National Food Safety Standard Food Additive Use Standard" stipulates the amount of cochineal added, which needs to be calculated as carminic acid. [0003] The detection methods of carminic acid reported so far mainly include spectrophotometry, thin layer chromatography and high performance liquid chromatography. In the detection process of spectrophotomet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D309/10B01D15/10B01D15/42G01N30/02G01N30/06G01N30/34
CPCB01D15/10B01D15/426C07D309/10G01N30/02G01N30/06G01N30/34
Inventor 杨祖伟苏杜威陈绮梦魏鲜娥李珍黄进丽吴小翠苏昭伦叶少文
Owner BY HEALTH CO LTD
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