Hydrogel, and preparation method and application thereof

A technology of hydrogel and gelatin solution, which can be applied in the direction of prosthesis, bandage, protein coating, etc., can solve problems such as difficult to apply, ignore material biocompatibility, complex and cumbersome material preparation process, etc., to achieve strong practicability and excellent Effect of biocompatibility and mechanical properties, good application effect

Pending Publication Date: 2020-02-14
SUN YAT SEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, related research work often pursues the mechanical strength of hydrogels one-sidedly (such as high elongation at break and breaking strength), but ignores the biocompatibility of the material, or the preparation process of the material itself is complicated and cumbersome, making it difficult to be practical.

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  • Hydrogel, and preparation method and application thereof
  • Hydrogel, and preparation method and application thereof
  • Hydrogel, and preparation method and application thereof

Examples

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preparation example Construction

[0051] In a specific embodiment, the preparation method of the hydrogel of the present invention comprises the following steps:

[0052] (1) Dissolving maleimidated alginate in phosphate buffer, and adjusting the pH value to 7.0-8.0 to obtain a maleimidated alginate solution; wherein, the maleimidated alginate Alginate is alginate modified by maleimidation, and the modification rate is 5-15%;

[0053] (2) dissolving the gelatin in hot phosphate buffer solution, and adjusting the pH value to 7.0-8.0 to obtain a gelatin solution;

[0054] (3) The maleimidated alginate solution is mixed with the gelatin solution to form a hydrogel mixed solution, which is solidified to obtain the hydrogel.

[0055] Gelatin is partially degraded from collagen in connective tissues such as animal skin, bone, and sarcolemma. There are a large number of hydroxyl groups in the molecular structure of gelatin, and there are also many carboxyl groups and amino groups, which make gelatin extremely hydro...

Embodiment 1

[0065] The preparation of maleimidated alginate comprises the steps:

[0066] (1) Dissolve 4.2g of alginate in 100mL of phosphate buffer, then add 1.38g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and 0.52g of N-hydroxysulfosuccinate imide, activated at 25°C for 1 hour;

[0067] (2) Add 0.51 g of N-(2-aminoethyl)maleimide trifluoroacetate to the activated solution in step (1), and magnetically stir at room temperature for 6 hours of coupling reaction;

[0068] (3) After the reaction, adjust the pH value of the solution to 6.0 with hydrochloric acid; transfer the solution to a dialysis bag, and perform dialysis in a dilute hydrochloric acid solution with a pH of 5.0 for 3 days, collect the dialysate in the dialysis bag after the dialysis, and freeze Drying gave purified maleimidated alginate as a white solid.

[0069] In the prepared maleimidated alginate, the modification rate of maleimidation was 5%. The H NMR spectrum of the prepared maleimidated alginate...

Embodiment 2

[0076] The preparation of maleimidated alginate comprises the steps:

[0077] (1) Dissolve 4.2g of alginate in 100mL of phosphate buffer, then add 2.76g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and 1.04g of N-hydroxysulfosuccinate imide, activated at 25°C for 1 hour;

[0078] (2) Add 1.02 g of N-(2-aminoethyl)maleimide trifluoroacetate to the activated solution in step (1), and magnetically stir at room temperature for 8 hours of coupling reaction;

[0079] (3) After the reaction is over, adjust the pH value of the solution to 5.0 with hydrochloric acid; transfer the solution to a dialysis bag, and perform dialysis in a dilute hydrochloric acid solution with a pH of 5.0 for 4 days, collect the dialysate in the dialysis bag after the dialysis, and freeze Drying gave purified maleimidated alginate as a white solid.

[0080] In the prepared maleimidated alginate, the maleimidated modification rate was 10%. The H NMR spectrum of the prepared maleimidated alg...

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Abstract

The invention provides hydrogel, and a preparation method and an application thereof. The hydrogel is hydrogel based on crosslinking of alginate and gelatin molecules, and has good biocompatibility; after the alginate is further subjected to maleimide modification, the crosslinking of the alginate and gelatin molecules can be tighter and more stable, so that the mechanical property of the hydrogelis further effectively improved on the premise of ensuring the biocompatibility of the hydrogel, and the hydrogel disclosed by the invention has excellent biocompatibility and mechanical property. The hydrogel has a good application effect in wound dressings and coating layers of implantable medical instruments, and the preparation method of the hydrogel is simple in operation procedure, high inrepeatability and prone to realization of industrialization, so that the hydrogel is high in practicability, and large-scale application of the hydrogel is facilitated.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a hydrogel and its preparation method and application. Background technique [0002] Biomedical materials are materials used to diagnose, treat, repair, or replace damaged tissues and organs of organisms. Its history can be traced back to around 3500 BC. The ancient Egyptians used cotton fibers and horsehair to suture wounds. In the following five thousand years, limited by the low productivity and scientific level of ancient civilizations, the development of biomedical materials was extremely slow, and humans could only carry out a small amount of simple medical applications through some easily obtained natural materials. For example, the Aztecs in ancient Mexico used wood chips to repair damaged skulls; while the Etruscans in ancient Rome carved animal bones into dentures and used gold for bridges and trays. During the two world wars in modern times, various new t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08J3/24C08L5/04C08L89/00C09D105/04C09D189/00A61L26/00A61L27/34A61L27/50A61L27/52
CPCA61L26/0023A61L26/0038A61L26/0061A61L26/008A61L27/34A61L27/50A61L27/52C08J3/075C08J3/246C08J2305/04C08J2389/00C08L2203/02C09D105/04C09D189/00C08L89/00C08L5/04
Inventor 陈寅周建华
Owner SUN YAT SEN UNIV
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