Method for reducing starch digestibility and application thereof

A digestibility and starch technology, applied in the field of enzyme engineering, can solve the problems of low production efficiency and low efficiency, and achieve the effect of high production efficiency

Active Publication Date: 2020-02-14
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low efficiency of existing enzymes such as maltose amylase, β-amylase, amylosucrase and / or glucosyltransferase to cleave starch and generate anti-digestion chemical bonds in starch, the use of enzymatic modification to prepare resistant Starch has the problem of low production efficiency

Method used

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  • Method for reducing starch digestibility and application thereof
  • Method for reducing starch digestibility and application thereof
  • Method for reducing starch digestibility and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: Preparation of different branching enzymes

[0056] Specific steps are as follows:

[0057] Chemical synthesis encodes the glycogen branching enzyme ChlGBE derived from Chlorella kessleri (the amino acid sequence is shown in SEQ ID NO: 1), and the glycogen branching enzyme HosGBE derived from Homo sapiens (the amino acid sequence is shown in SEQ ID NO: 1). ID NO: 2), glycogen branching enzyme VvGBE derived from Vibrio vulnificus (amino acid sequence is shown in SEQ ID NO: 3), starch branching enzyme derived from cyanobacteria (Cyanothece sp.ATCC 51142) CySBE (amino acid sequence shown in SEQ ID NO: 4) or the gene of starch branching enzyme RoSBE (amino acid sequence shown in SEQ ID NO: 5) derived from Rhodothermus obamensis (nucleotide sequence, respectively) (As shown in SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10), and connect the synthesized gene and pET-24a(+) plasmid , Get the recombinant plasmid pET-24a-ChlGBE, pET-24a-HosGBE, pET-24a-...

Embodiment 2

[0059] Example 2: The ability of different branching enzymes to reduce starch digestibility

[0060] Specific steps are as follows:

[0061] Weigh 2.5g of corn starch and add 50mM, pH 7.0 sodium dihydrogen phosphate-disodium hydrogen phosphate buffer to obtain a starch solution; heat and stir the starch solution in a boiling water bath for 30 min. After heating, use a volumetric flask to dilute to 100 mL , Prepared into a 2.5% gelatinized starch solution; place the gelatinized starch solution in a 30°C, 150rpm constant temperature water bath shaker for 10 minutes to obtain an incubation starch solution; add 100, 200, 500, and 1000 U to the incubation starch solution, respectively / g starch of the crude enzyme solution of glycogen branching enzyme ChlGBE, glycogen branching enzyme HosGBE, glycogen branching enzyme VvGBE, branching enzyme CySBE or branching enzyme RoSBE obtained in Example 1, wherein the blank control group (Control) does not add enzymes Make up the solution with bu...

Embodiment 3

[0066] Example 3: Effect of reaction time on the ability of branching enzymes to reduce starch digestibility

[0067] Weigh 2.5g of corn starch and add 50mM, pH 7.0 sodium dihydrogen phosphate-disodium hydrogen phosphate buffer to obtain a starch solution; heat and stir the starch solution in a boiling water bath for 30 min. After heating, use a volumetric flask to dilute to 100 mL , Prepared into a 2.5% gelatinized starch solution; place the gelatinized starch solution in a constant temperature water bath shaker at 30° C. and 150 rpm for 10 minutes to obtain an incubated starch solution; examples of adding 500 U / g starch to the incubating starch solution 1 The obtained crude enzyme solution of glycogen branching enzyme ChlGBE, glycogen branching enzyme HosGBE or glycogen branching enzyme VvGBE was reacted at 30°C for 0, 4, 8, 10, 12 h, and the reaction was terminated by heating and boiling off the enzyme to obtain The reaction solution. The content of resistant starch (RS) in t...

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Abstract

The invention discloses a method for reducing starch digestibility and application thereof, and belongs to the technical field of enzyme engineering. The invention provides a method for reducing the starch digestibility, and the method can be used for preparing resistant starch and has very high production efficiency. By utilizing the method, a glycogen branching enzyme ChlGBE is added into a starch solution to be modified for 6 hours, so that the content of resistant starch in the starch solution can be as high as 35.06% and is increased by 9.75% compared with that of an unmodified starch solution. By utilizing the method, a glycogen branching enzyme HosGBE is added into a starch solution to be modified for 8 hours, so that the content of resistant starch in the starch solution can be ashigh as 47.36% and is increased by 22.05% compared with that of an unmodified starch solution. By utilizing the method, a glycogen branching enzyme VvGBE is added into a starch solution to be modifiedfor 10 hours, so that the content of resistant starch in the starch solution can be as high as 51.4% and is increased by 26.09% compared with that of an unmodified starch solution.

Description

Technical field [0001] The invention relates to a method and application for reducing starch digestibility, and belongs to the technical field of enzyme engineering. Background technique [0002] Starch is the main carbon storage and energy storage polysaccharide in nature. It can provide 70-80% of the energy for the human body and is the most important source of dietary energy for the human body. According to different digestibility, starch can be divided into three types, namely fast digestion starch (RDS), slow digestion starch (SDS) and resistant starch (RS). Among them, fast-digestible starch (RDS) refers to the starch that is completely digested and absorbed in the small intestine, slow-digested starch refers to the starch that is completely digested and absorbed in the small intestine 20 to 120 minutes, and resistant starch refers to the starch that cannot be digested and absorbed by the small intestine after 120 minutes. starch. [0003] Studies have shown that due to its...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/18C12P19/04
CPCC12P19/04C12P19/18
Inventor 吴敬宿玲恰李玲玲李娜
Owner JIANGNAN UNIV
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