Primer, reagent kit and detection method for detecting human CYP2C19 gene mutation

Abstract

The invention relates to a primer, reagent kit and detection method for detecting human CYP2C19 gene mutation. The primer comprises a primer and probe for detecting No.5 exon mutation of a CYP2C19*2 equipotential gene, and a primer and probe for detecting No.4 exon mutation of a CYP2C19*3 equipotential gene. According to the method, negative control and positive control are set, and false positiveresults caused by pollution can be effectively avoided, and whether the amplification reagent quality achieves a set standard or not is evaluated. External control reaction liquid can accurately reflect the quality of a formwork to be tested, the false positive results caused by formwork number insufficiency or too high inhibitors can be avoided, and false positives which can be caused due to high formwork number can also be monitored. Besides, an internal reference primer and probe is added to the mutation detection reaction liquid, and through amplification of internal reference, the generation of the false positives can be effectively avoided, and finally, accurate testing results of the reagent kit can be guaranteed. The reagent kit and the detection method are high in sensitivity andhigh in specificity, and can guide various symptom patients to realize personalized medication according to individual genome information.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically, the invention relates to a primer, a kit and a detection method for detecting human CYP2C19 gene mutation. Background technique [0002] Pharmacodynamics is a science that studies the effects, rules and mechanisms of drugs on the body. The target works. The detection of drug-metabolizing enzymes and drug target genes can guide clinical selection of appropriate drugs and dosages for specific patients, and realize individualized drug use, thereby improving the effectiveness and safety of drug treatment and preventing the occurrence of serious adverse drug reactions . [0003] In recent years, with the development of human genomics, the field of pharmacogenomics has developed rapidly, and more and more pharmacogenomic biomarkers and their detection methods have emerged one after another. Pharmacogenomics has become an important tool to guide clinical individualized medication, ...

AI Technical Summary

Problems solved by technology

[0005] At present, the commonly used CYP2C19 gene mutation detection method mainly uses the Taqman fluorescent probe method, but the Taqman fluorescent probe method has low sensitivity, high false positive rate, long time-consuming, and low throughput

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