Application of corn flour in improving quality of mulberry twig and leaf silage
A technology of green branches and leaves and corn flour, which is applied in the fields of application, animal feed raw material preservation methods, animal feed, etc., can solve the problems of slow pH drop, multiplication of miscellaneous bacteria, unfavorable long-term reproduction of lactic acid bacteria, etc., to improve color and improve silage environmental effects
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Embodiment 1
[0039] 1 Materials and methods
[0040] 1.1 Test material
[0041] Mulberry branches and leaves: planted in the feed mulberry base of Chongqing Academy of Animal Sciences, the variety is Guisangyou 62.
[0042] Corn flour: raw corn flour, purchased from RT-Mart Supermarket, No. 23, Baorong Road, Rongchang District, sterilized in a constant temperature drying oven at 95°C for 4 hours, and the amount of silage added was 90g / repeat.
[0043] Lactobacillus plantarum: purchased from China Agricultural Microorganism Culture Collection Center (ACCC), preservation number: ACCC11016, prepared 4.30×10 9 CFU / ml bacterial solution, the amount of silage added is 0.5ml / repeat.
[0044] Lactobacillus Brucella: purchased from China Industrial Microorganism Culture Collection Center (CICC), preservation number: 20293, prepared 6.32×10 8 CFU / ml bacterial solution, the amount of silage added is 0.5ml / repeat.
[0045] 1.2 Experimental design
[0046] The method of cutting twigs from mulberry...
Embodiment 2
[0097] 1 Materials and methods
[0098] 1.1 Test material
[0099] See 1.1 and 1.3 of Example 1
[0100] 1.2 Main instruments and reagents
[0101] 1.2.1 Main instruments
[0102] Century Ark pHS-3C+, constant temperature blast drying oven, vacuum pump, Shimadzu high performance liquid chromatography LC-20A liquid chromatograph, ultrasonic oscillator, constant temperature oscillator, ultraviolet spectrophotometer, FOSS fat extractor (Soxhlet extractor) ), FOSS fiber tester, VELP automatic Kjeldahl nitrogen analyzer and constant temperature water bath.
[0103] 1.2.2 Main reagents
[0104] 0.45μm synthetic cellulose ester film: Tianjin Jinteng Experimental Equipment Co., Ltd.; lactic acid standard: Beijing Suolaibao Technology Co., Ltd., purity > 98%; acetic acid standard: EhrenstorferQualityDr.Ehrenstorfer, purity > 99%; propionic acid standard Product: Ehrenstorfer Quality Dr. Ehrenstorfer, purity > 98%; Butyric acid standard: Ehrenstorfer Quality Dr. Ehrenstorfer, purit...
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