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Microbial remediation agent based on bacterial strain 1JN2, and preparation and application methods thereof

A microbial remediation, 1JN2 technology, applied in the field of microbial remediation agents, can solve problems such as soil heavy metal pollution

Active Publication Date: 2020-02-28
HUAIYIN TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a microbial remediation agent based on bacterial strain 1JN2, which can fundamentally solve the problem of soil heavy metal pollution, and its preparation method is simple and convenient, with low cost, and is convenient for mass production and preparation

Method used

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  • Microbial remediation agent based on bacterial strain 1JN2, and preparation and application methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take 50kg of microbial restoration agent based on strain 1JN2 as an example:

[0023] S1. Shake 50ml of the stress-resistant strain 1JN2 in 250ml of LB medium at 28°C and 180rpm for 16h, then centrifuge at 6000rpm for 10min, resuspend and dilute with 5500ml of sterilized water, add sterilized water to quantify to 6000ml, and obtain the stress-resistant strain agent;

[0024] S2. Mix and stir 5362ml of sterilized water, 120g of brown sugar, 12g of yeast extract powder and 6g of trace elements to obtain a culture medium, then add 300ml of the total weight of the culture medium to the EM bacteria stock solution, and place it in a fermenter at 30-35°C Cultivate for 2 days, add sterilized water to quantitatively reach 6000ml, and obtain EM bacterial agent;

[0025] S3. Mix 50 kg of crushed corn stalks and 50 kg of wheat stalks and place them in a muffle furnace, anoxic carbonization at 550° C. for 2 hours, and take out the black residue after the muffle furnace is cooled to...

Embodiment 2

[0030] Take 50kg of microbial restoration agent based on strain 1JN2 as an example:

[0031] S1. Shake 50ml of the stress-resistant strain 1JN2 in 250ml of LB medium at 28°C and 180rpm for 16h, then centrifuge at 6000rpm for 10min, resuspend and dilute with 5500ml of sterilized water, add sterilized water to quantify to 6000ml, and obtain the stress-resistant strain agent;

[0032] S2. Mix and stir 5362ml of sterilized water, 120g of brown sugar, 12g of yeast extract powder and 6g of trace elements to obtain a culture medium, then add 300ml of the total weight of the culture medium to the EM bacteria stock solution, and place it in a fermenter at 30-35°C Cultivate for 2 days, add sterilized water to quantitatively reach 6000ml, and obtain EM bacterial agent;

[0033] S3. Mix 50 kg of crushed corn stalks and 50 kg of wheat stalks and place them in a muffle furnace, anoxic carbonization at 550° C. for 2 hours, and take out the black residue after the muffle furnace is cooled to...

Embodiment 3

[0038] Take 50kg of microbial restoration agent based on strain 1JN2 as an example:

[0039] S1. Shake 50ml of the stress-resistant strain 1JN2 in 250ml of LB medium at 28°C and 180rpm for 16h, then centrifuge at 6000rpm for 10min, resuspend and dilute with 5500ml of sterilized water, add sterilized water to quantify to 6000ml, and obtain the stress-resistant strain agent;

[0040] S2. Mix and stir 5362ml of sterilized water, 120g of brown sugar, 12g of yeast extract powder and 6g of trace elements to obtain a culture medium, then add 300ml of the total weight of the culture medium to the EM bacteria stock solution, and place it in a fermenter at 30-35°C Cultivate for 2 days, add sterilized water to quantitatively reach 6000ml, and obtain EM bacterial agent;

[0041] S3. Mix 50 kg of crushed corn stalks and 50 kg of wheat stalks and place them in a muffle furnace, anoxic carbonization at 550° C. for 2 hours, and take out the black residue after the muffle furnace is cooled to...

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Abstract

The invention relates to a microbial remediation agent based on a bacterial strain 1JN2. The microbial remediation agent comprises the following raw materials in parts by weight: 12-15 parts of biochar, 3-6 parts of a silkworm excrement organic fertilizer, 3-5 parts of a composite microbial agent, 0.5 to 1 part of amino acid, 0.5 to 1 part of a biological growth promoter and 0.5-1 part of a slow release oxidant, wherein the composite microbial agent comprises a stress-resistant strain 1JN2 and EM bacteria. The microbial remediation agent is prepared through the following steps: respectively preparing two microbial agents, mixing two microbial agents with the biochar for fermentation, and mixing an obtained material with the silkworm excrement organic fertilizer, the amino acid, the biological growth promoter and the slow release oxidant. The application method comprises the following steps: spreading the microbial remediation agent based on the bacterial strain 1JN2 onto the surface layer of to-be-treated soil, deeply ploughing the soil to a depth of 500 mm by using an excavator, respectively ploughing the soil twice in a longitudinal direction and a transverse direction, and covering the soil with a layer of thin sandy soil. With the microbial remediation agent based on the bacterial strain 1JN2, and the preparation method and the application method thereof in the above-mentioned technical scheme of the invention, the problem of heavy metal pollution of the soil can be fundamentally solved; and the preparation method is simple and convenient, has low cost and facilitates large-scale production and preparation.

Description

technical field [0001] The invention relates to the technical field of microbial remediation agents, in particular to a microbial remediation agent for treating heavy metal-contaminated soil, a preparation method and a use method thereof. Background technique [0002] Soil heavy metal pollution has become one of the important global environmental problems. Once the land is polluted by heavy metals, it will lose its productive capacity to a certain extent. For soil pollution treatment, the biggest disadvantage of traditional physical and chemical remediation technologies is that the removal of pollutants is not complete, which easily leads to secondary pollution, which brings a certain degree of environmental health risks. Bioremediation of different types of contaminated soils by using microorganisms has become one of the hotspots in microbial research. Microbial remediation refers to the bioremediation technology that uses naturally occurring or cultivated functional micro...

Claims

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Application Information

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IPC IPC(8): C09K17/40C12N1/20B09C1/10B09C1/00A01B79/00C12R1/125
CPCA01B79/00B09C1/00B09C1/10B09C1/105C09K17/40C12N1/20
Inventor 杨威闫海霞罗玉明谷益安黄如江高繁
Owner HUAIYIN TEACHERS COLLEGE
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