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Method for identifying cryptococcus neoformans and cryptococcus gattii by utilizing Raman scattering

A technology of Cryptococcus neoformans and Raman scattering method, which is applied in the field of clinical microbiology inspection, can solve the problems of high cost of equipment and difficulty in clinical practice, and achieve the effect of enhancement

Active Publication Date: 2020-02-28
ACADEMY OF MILITARY MEDICAL SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

For example, although gene chip technology has the advantages of high throughput, rapidity and automation, the cost of equipment is high and it is difficult to be used in clinical practice.

Method used

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  • Method for identifying cryptococcus neoformans and cryptococcus gattii by utilizing Raman scattering
  • Method for identifying cryptococcus neoformans and cryptococcus gattii by utilizing Raman scattering
  • Method for identifying cryptococcus neoformans and cryptococcus gattii by utilizing Raman scattering

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Embodiment 1

[0029] 1. Reagents

[0030] Silver Nitrate (AgNO 3 ): Beijing Modern Oriental Company Trading Co., Ltd.; sodium borohydride (NaBH 4 ): Sigma-Aldrich (Shanghai) Trading Co., Ltd.; Cetyltrimethylammonium Bromide (CTAB) Sigma-Aldrich (Shanghai) Trading Co., Ltd.; Ammonium Hydroxide (NH 4 OH): Xilong Chemical Group Co., Ltd.; monocrystalline silicon wafers: Zhejiang Lijing Silicon Materials Co., Ltd.; deionized water: Milli-Q pure water instrument Sa Paul medium: Shanghai Kemajia Microbial Technology Co., Ltd.

[0031] 2. Instrument

[0032] Hitachi H-7650 Transmission Electron Microscope: Hitachi, Japan; JEOL 2010 High Resolution Transmission Electron Microscope: JEOL Co., Ltd.; i-Raman Plus BWS465-785H Raman Spectrometer: B&W Tek Zeta potential was measured using Zetasizer analyzer (Zetasizer Nano ZSP, Malvern Inc ., UK; Shimadzu 2600 UV-Visible Spectrophotometer Shimadzu, Japan; MiniSpin centrifuge: Germany; Eppendorf ZNCL-BS230*230 heating electromagnetic stirrer: Beijing Century Hua...

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Abstract

The invention discloses a method for identifying cryptococcus neoformans and cryptococcus gattii by utilizing Raman scattering. According to the invention, distinguishing cryptococcus neoformans and cryptococcus gattii is taken as an entry point, and a detection method capable of enhancing Raman spectrum signals of the two fungi and distinguishing the two fungi is developed in a targeted manner. Results show that the surfaces of the two fungi are negatively charged, positive-valence colloidal silver can be well adsorbed, Raman detection signals of the fungi can be well enhanced by developing the positive-valence colloidal silver with a proper particle size, the fungi extremely close to the fungi can be identified, the method and the material are expected to be applied to more fungus detection, and development of clinical fungus identification is promoted.

Description

Technical field [0001] The invention belongs to the technical field of clinical microbiological testing, and specifically relates to a method for identifying Cryptococcus neoformans and Cryptococcus guttata using Raman scattering. Background technique [0002] Cryptococcus neoformans and Cryptococcus gordonis are the two main pathogenic bacteria that cause human infections. Cryptococcus neoformans causes most cryptococcal infections. Since Gatti and Eeckels reported the world's first case of Cryptococcus guttata in 1970, related infections began to be reported more frequently. Initially, Cryptococcus gedetii was called Cryptococcus neoformans var. guttata. In 2002, Kwon-Chung et al. named the C. neoformans variant of Cryptococcus garethi based on differences in genetic taxonomy, biology and phenotype. Cryptococcus neoformans mainly infects immunosuppressed people and often causes central nervous system infections, while Cryptococcus guttata mainly infects people with normal im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65B82Y40/00
CPCG01N21/658B82Y40/00Y02A50/30
Inventor 杨英顾兵胡珊顾锋杨健王升启
Owner ACADEMY OF MILITARY MEDICAL SCI
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