Anti-renal fibrosis medicine and preparation method thereof
A kidney fibrosis and drug technology, applied in the field of drugs for treating renal fibrosis and their preparation, can solve the problems of poor specificity and poor efficacy of western medicines
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Embodiment 1
[0075] Based on high-throughput sequencing technology, the regulation of 35 kinds of traditional Chinese medicine ethanol extracts on five key genes of renal fibrosis in human renal tubular epithelial cells HK2 was detected.
[0076] 1. Preparation of single herbal extracts of 35 kinds of Chinese herbal medicines:
[0077] Atractylodes macrophylla, puffball, spider plant, cicada slough, snake slough, shegan, peach branch, pterygium, duckweed, Cimicifuga, white jelly, sinew grass, saponin thorn, neem bark, light bamboo leaf, Tongcao, Changshan, red flower dragon Gallbladder, Xu Changqing, Ligustrum lucidum, Sijiqing, Spatholobus yunnanensis, Nuxychia chinensis, lotus root, three needles, Shannai, Eucommia, loquat leaves, cnidium, licorice, Eucommia leaves, astragalus, ginseng, motherwort, peony Skin.
[0078] Take the above Chinese medicinal materials, crush them into 80-100 mesh powder, add 15 times the volume of 90% ethanol, standard Soxhlet reflux extraction for 3 hours, co...
Embodiment 2
[0086] Take the composition of Atractylodes macrocephala 2.5g, puffball 2.5g, spider plant 2.5g, and cicada slough 2.5g as examples to verify the efficacy of the traditional Chinese medicine composition:
[0087] 1. Extraction of the composition:
[0088] Crush the formula and weigh it as 80-100 mesh powder, weigh it according to the proportion, mix it, add 15 times the volume of 90% ethanol, standard Soxhlet reflux extraction for 3 hours, concentrate the extract for 20 minutes to a volume of 2 mL, and use a lyophilizer to lyophilize under reduced pressure After 24 hours, the extract was made into freeze-dried powder, and a certain quality of the freeze-dried powder was weighed, dissolved in DMSO to make the mother liquor of the extract, and stored at -20°C.
[0089] 2. Regulation of the extract on epithelial-mesenchymal transition of human renal tubular cells
[0090] 2.1 HK2 cells were plated in 12-well plates at 80,000 / well. After overnight, serum was starved for 24 hours,...
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