Anti-renal fibrosis medicine and preparation method thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A kidney fibrosis and drug technology, applied in the field of drugs for treating renal fibrosis and their preparation, can solve the problems of poor specificity and poor efficacy of western medicines
Active Publication Date: 2020-03-06
CAPITALBIO CORP +1
View PDF1 Cites 6 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0003] At present, the specificity of western medicine for anti-renal fibrosis is not strong, and the curative effect is not good.
The traditional Chinese medicine pathogenesis of renal fibrosis is deficiency in origin and excess in superficiality. The deficiency in origin is mainly the deficiency of kidney qi, and the excess in superficiality mainly refers to blood stasis, damp heat, and flooding. Traditional Chinese medicine has unique clinical advantages in treating renal fibrosis, but it is still No specific traditional Chinese medicine composition can effectively treat renal fibrosis
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0075] Based on high-throughput sequencing technology, the regulation of 35 kinds of traditional Chinese medicine ethanol extracts on five key genes of renal fibrosis in human renal tubular epithelial cells HK2 was detected.
[0076] 1. Preparation of single herbal extracts of 35 kinds of Chinese herbal medicines:
[0078] Take the above Chinese medicinal materials, crush them into 80-100 mesh powder, add 15 times the volume of 90% ethanol, standard Soxhlet reflux extraction for 3 hours, co...
Embodiment 2
[0086] Take the composition of Atractylodes macrocephala 2.5g, puffball 2.5g, spider plant 2.5g, and cicada slough 2.5g as examples to verify the efficacy of the traditional Chinese medicine composition:
[0087] 1. Extraction of the composition:
[0088] Crush the formula and weigh it as 80-100 mesh powder, weigh it according to the proportion, mix it, add 15 times the volume of 90% ethanol, standard Soxhlet reflux extraction for 3 hours, concentrate the extract for 20 minutes to a volume of 2 mL, and use a lyophilizer to lyophilize under reduced pressure After 24 hours, the extract was made into freeze-dried powder, and a certain quality of the freeze-dried powder was weighed, dissolved in DMSO to make the mother liquor of the extract, and stored at -20°C.
[0089] 2. Regulation of the extract on epithelial-mesenchymal transition of human renal tubular cells
[0090] 2.1 HK2 cells were plated in 12-well plates at 80,000 / well. After overnight, serum was starved for 24 hours,...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention relates to the technical field of traditional Chinese medicines, in particular to a medicine for treating renal fibrosis and a preparation method thereof. The 31 traditional Chinese medicinal materials provided by the invention have the effects of anti-renal fibrosis, anti-heart-kidney syndrome, anti-heart failure, anti-nephrotic syndrome and/or anti-renal failure medicines; a composition prepared from rhizoma atractylodis macrocephalae, puffball, lysionotus pauciflorus and periostracum cicadae can inhibit renal tubular cell epithelial-mesenchymal transition and inhibit the transcription level of key genes, and therefore renal fibrosis, heart-kidney syndrome, heart failure, nephrotic syndrome and renal failure can be effectively treated.
Description
technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a medicine for treating renal fibrosis and a preparation method thereof. Background technique [0002] Renal fibrosis is the common pathological basis for the development of various renal diseases to end-stage renal failure, and is an important pathological process of cardiorenal syndrome and heart failure. The development of new therapies for renal fibrosis is of great significance for renal failure and cardiorenal syndrome. Renal fibrosis is associated with dysregulated expression of various genes, including human plasminogen activator inhibitor 1 gene (SERPINE1), type Ⅰ collagen α1 gene (COL1A1), human interleukin 13 receptor α1 (IL13RA1), endothelin receptor B(EDNRB) and SMAD4 are key genes affecting renal fibers. Inhibiting the key genes of renal fibrosis can effectively inhibit the pathological process of renal fibrosis, prevent the accumulation of ex...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more