Method for extracting chitin from litopenaeus vannamei shells
A technology for chitin and prawns, applied in the biological field, can solve the problems of low efficiency, low decalcification rate and deproteinization rate, etc., and achieve the effect of high yield and high purity
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Embodiment 1
[0021] 1) Preparation of Litopenaeus vannamei shell powder: firstly, the shells of Litopenaeus vannamei were cleaned with distilled water, and then dried in an oven for two days. Then crush the dried shrimp shells to 0.5-1.0 mm with a pulverizer, and set aside;
[0022] 2) Preparation of the strain seed solution: activate Lactobacillus rhamnosus tangarus in a conventional manner, and inoculate it into MRS medium for 12 hours after activation; activate Bacillus amyloliquefaciens in a conventional manner, and inoculate it into LB culture after activation cultured in medium for 12 hours.
[0023] 3) Inoculate the seed liquid of Lactobacillus rhamnosus in the above step 2) into 5 g of the above step 1) shrimp shell powder and 5 g of glucose mixture with an inoculation amount of 4% by volume; adjust the system to pH6.5, and then Fermented and cultured at 37°C for 48 hours; then the fermentation broth was centrifuged at 4000rpm for 10 minutes to collect the precipitate. At this tim...
Embodiment 2
[0030] 1) Preparation of Litopenaeus vannamei shell powder: firstly, the shells of Litopenaeus vannamei were cleaned with distilled water, and then dried in an oven for two days. Then crush the dried shrimp shells to 0.5-1.0 mm with a pulverizer, and set aside;
[0031] 2) Preparation of the strain seed solution: activate Lactobacillus rhamnosus tangarus in a conventional manner, and inoculate it into MRS medium for 12 hours after activation; activate Bacillus amyloliquefaciens in a conventional manner, and inoculate it into LB culture after activation cultured in medium for 12 hours.
[0032] 3) Inoculate the Lactobacillus rhamnosus seed solution in the above step 2) into 5 g of the above step 1) shrimp shell powder and 5 g of glucose mixture with an inoculation amount of 2% by volume; adjust the system to pH6.5, and then Ferment and cultivate at 37°C for 48 hours; then centrifuge the fermentation broth at 4000rpm for 10 minutes to collect the precipitate. At this time, the ...
Embodiment 3
[0035] 1) Preparation of Litopenaeus vannamei shell powder: firstly, the shells of Litopenaeus vannamei were cleaned with distilled water, and then dried in an oven for two days. Then crush the dried shrimp shells to 0.5-1.0 mm with a pulverizer, and set aside;
[0036] 2) Preparation of the strain seed solution: activate Lactobacillus rhamnosus tangarus in a conventional manner, and inoculate it into MRS medium for 12 hours after activation; activate Bacillus amyloliquefaciens in a conventional manner, and inoculate it into LB culture after activation cultured in medium for 12 hours.
[0037] 3) Inoculate the Lactobacillus rhamnosus seed liquid in the above step 2) into 1000g of the above step 1) shrimp shell powder and 1000g glucose mixture with an inoculation amount of 4% by volume; adjust the system to pH 6.5, and then 37°C, ferment and cultivate for 48 hours; then centrifuge the fermented liquid at 4000rpm for 10 minutes to collect the precipitate. At this time, the remo...
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