Plant senescence-related protein GhWRKY91 as well as coding gene and application thereof

A plant senescence and plant technology, applied to the plant senescence-related protein GhWRKY91 and its coding gene and application field, can solve the problems of short-season cotton premature senescence, etc., and achieve the effect of great application value

Active Publication Date: 2020-03-10
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The contradiction between grain and cotton competing for land in our country is prominent, and the selection and promotion of short-season cotton varieties can alleviate the contradiction of grain and cotton competing for land, but the early maturity of short-season cotton is often accompanied by premature aging

Method used

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  • Plant senescence-related protein GhWRKY91 as well as coding gene and application thereof
  • Plant senescence-related protein GhWRKY91 as well as coding gene and application thereof
  • Plant senescence-related protein GhWRKY91 as well as coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0061] 1. Construction of recombinant expression vector

[0062] 1. Extract the mature leaves of the upland cotton Zhongmiansuo No. 74 plant at the full flowering stage, and extract the total RNA.

[0063] 2. Reverse transcribe the total RNA obtained in step 1 to obtain cDNA.

[0064] 3. Using the cDNA obtained in step 2 as a template, perform PCR amplification with a primer pair composed of F1 and R1, and recover the PCR amplification product.

[0065] F1: 5'-CTAG TCTAGA ATGGACAGCGGTGGTAG-3';

[0066] R1: 5'-TCC CCCGGG TCAGCAGATCCCAACCTG-3'.

[0067] 4. Digest the PCR amplified product obtained in step 3 with restriction endonucleases XbaI and SmaI, and recover the digested product.

[0068] 5. Digest the pBI121 plasmid with restriction endonucleases XbaI and SmaI to recover the vector backbone.

[0069] 6. Ligate the digested product obtained in step 4 with the vector backbone obtained in step 5 to obtain recombinant plasmid pBI121-GhWRKY91. According to the sequenc...

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Abstract

The invention discloses a plant senescence-related protein GhWRKY91 as well as a coding gene and application thereof. The protein provided by the invention is a protein shown in a sequence 1 in a sequence table. The nucleic acid molecule encoding the protein belongs to the protection scope of the invention. The invention also provides a protection range for the following application of the protein: regulating a plant senescence process; regulating a plant leaf senescence process; inhibiting the plant senescence process; suppressing the plant leaf senescence process; regulating the plant senescence process in a drought stress environment; regulating the plant leaf senescence process in the drought stress environment; regulating the plant drought resistance; and improving the plant drought resistance. The invention also provides a protection range for a method for preparing a transgenic plant; and the method comprises the following step: introducing the gene into a starting plant to obtain the transgenic plant with a delayed senescence process. The protein and gene provided by the invention have great theoretical value for the study of a plant senescence mechanism, and have great application value for cultivating anti-premature senescence plants, especially anti-premature senescence cotton.

Description

technical field [0001] The invention relates to a plant aging-related protein GhWRKY91 and its coding gene and application. Background technique [0002] Senescence is an integral part of plant development and is the final stage of development. During aging, leaf cells undergo highly coordinated changes in structure, metabolism, and gene expression. The earliest and most visible change in cellular structure is the breakdown of chloroplasts. In terms of metabolism, carbon metabolism (photosynthesis) is replaced by catabolism of chloroplasts and macromolecular substances such as proteins, membrane lipids, RNA, etc. At the molecular level, the changes described above are accompanied by changes in gene expression. [0003] Senescence is programmed cell death. Leaf senescence plays an important role in physiological activities such as recycling and reutilization of nutrient elements. Leaf assimilation function decline caused by senescence limits crop yield. [0004] Cotton ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/20A01H6/60
CPCC07K14/415C12N15/8273C12N15/8261
Inventor 喻树迅顾丽姣魏恒玲王寒涛王聪聪马亮苏政政
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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