Induction method of lysozyme amyloid fiber model

Abstract

The invention discloses an induction method of a lysozyme amyloid fiber model. The induction method comprises the following steps: (1) selecting lysozyme; (2) preparing an induction buffer solution; (3) preparing a lysozyme solution with a certain concentration; and (4) inducing at different temperatures. According to the method, high-activity lysozyme is dissolved in a glycine-hydrochloric acid buffer system, strong acid conditions are artificially created, and conversion of a lysozyme protein secondary structure is facilitated; during high-temperature induction, protein can be mistakenly folded to form amyloid fiber aggregates, the amyloid fiber model can be better built, the success rate of lysozyme amyloid fibrillation can be increased, and the obtained fibers are higher in toughness.Experiment errors caused by environmental problems can be reduced, no batch difference is caused by the induction method, repeatability and stability of data obtained through the model are improved, and the method is simple and beneficial to popularization.

Description

technical field [0001] The invention relates to the field of constructing simulated disease models, in particular to a method for inducing a lysozyme amyloid fiber model. Background technique [0002] The main pathological process of neurodegenerative diseases such as Alzheimer's disease (AD) and Parkinson's disease (PD) is the misfolding of related proteins to form amyloid fibers, which accumulate in the central nervous system. system, leading to neuronal cell damage, changes in signal transduction, and even neuron loss. It is particularly important to study the mechanism of neurodegenerative diseases, the conditions for the formation of protein amyloid fibrils, and the formation process. However, due to the high cost of protein materials used to study the formation of amyloid fibrils, and the uncertainty of the formation process and conditions of amyloid fibrils, it brings huge economic risks to scientific research. Low-cost protein materials are used as a substitute to ...

AI Technical Summary

Problems solved by technology

However, egg white lysozyme is a normal protein, and the amyloid fibers formed are not toxic in vivo, which has become a deficiency and defect in the in vivo evaluation process