Induction method of lysozyme amyloid fiber model
A fiber model and amyloid technology, applied in biochemical equipment and methods, enzymes, hydrolytic enzymes, etc., can solve the problem of non-toxicity of amyloid fibers, and achieve the effect of prolonging experimental operability, saving costs, and prolonging operation time
Pending Publication Date: 2020-03-24
SHAANXI SCI TECH UNIV
5 Cites 0 Cited by
AI-Extracted Technical Summary
Problems solved by technology
View more
Abstract
The invention discloses an induction method of a lysozyme amyloid fiber model. The induction method comprises the following steps: (1) selecting lysozyme; (2) preparing an induction buffer solution; (3) preparing a lysozyme solution with a certain concentration; and (4) inducing at different temperatures. According to the method, high-activity lysozyme is dissolved in a glycine-hydrochloric acid buffer system, strong acid conditions are artificially created, and conversion of a lysozyme protein secondary structure is facilitated; during high-temperature induction, protein can be mistakenly folded to form amyloid fiber aggregates, the amyloid fiber model can be better built, the success rate of lysozyme amyloid fibrillation can be increased, and the obtained fibers are higher in toughness.Experiment errors caused by environmental problems can be reduced, no batch difference is caused by the induction method, repeatability and stability of data obtained through the model are improved, and the method is simple and beneficial to popularization.
Application Domain
Glycosylases
Technology Topic
Protein proteinBiophysics +11
Examples
- Experimental program(1)
Example Embodiment
PUM


Description & Claims & Application Information
We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.
Similar technology patents
Magnetically guided catheter
Owner:ST JUDE MEDICAL ATRIAL FIBRILLATION DIV
Method for treating a solid material to make it hydrophobic, material obtained and uses
Owner:BT3 TECH
OLED apparatus having improved fault tolerance
Owner:GLOBAL OLED TECH
Material including graphene and an inorganic material and method of manufacturing the material
Owner:SAMSUNG ELECTRONICS CO LTD +1
Preparing method of bamboo fiber winding composite tube
Owner:XINJIANG GUANGSHUI PIPE
Classification and recommendation of technical efficacy words
- save electricity
- low cost
Gyro-type dynamic self-balancing pan-tilt
Owner:SZ DJI OSMO TECH CO LTD
Novel megawatt wind generating set testing system and testing method thereof
Owner:INNER MONGOLIA GEOHO ENERGY EQUIP
Energy feeding type electrical energy mass perturbance generating device
Owner:SOUTHEAST UNIV
Food baking one-key-system control method
Owner:LG ELECTRONICS (TIANJIN) APPLIANCES CO LTD