Aquatic product composite biological fresh-keeping agent as well as preparation method and application thereof
A technology for biological preservatives and aquatic products, which is applied in the preservation and application of food ingredients as antimicrobials, and the preservation of meat/fish with chemicals, etc. The preparation method is scientific, the ingredients are clear, and the effect of increasing the freshness
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[0030] A preparation method of a composite biological preservative for aquatic products, comprising the following steps:
[0031] (1) Material pretreatment: select fresh plants of high-quality Salicornia, dry them in a blast drying oven at 55 degrees Celsius after collection, and crush them after drying to obtain powders with different crushing degrees (crushing degree ≥ 60 mesh);
[0032] (2) Extraction of active ingredients of Salicornia: Clean the extraction tank, add 10-12 times the amount (mass ratio) of 70% ethanol to soak at room temperature for 2 hours, heat and reflux extraction for 3 times, each time for 2-4 hours, Recover the extract, concentrate the extract in the filtrate under reduced pressure at 55°C to 0.2~0.5g / mL (calculated as crude drug);
[0033] (3) Column chromatography: the concentrated extract was enriched with macroporous adsorption resin, 100 g of AB-8 macroporous adsorption resin was selected, first soaked overnight in 95% ethanol, packed in a wet co...
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