Insulin releasing and recovering method and device and control system of insulin releasing and recovering device
A recovery system, insulin technology, applied in the field of insulin release and recovery, to achieve the effect of prolonging the use cycle, avoiding damage and increasing availability
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Embodiment 1
[0044] Example 1: Preparation method of magnetically labeled insulin
[0045] In the present invention, we used EDC / NHS to activate the surface carboxyl-modified MNPs to react with the amino groups of insulin and achieved good results. Here's how we label insulin with magnetic nanoparticles.
[0046] (1) Dissolve 8 mg of EDC and 1 mg of NHS in 2 mL of deionized water, add 2 mL of carboxylated Fe3O4 nanoparticles, and stir at room temperature for 20 min.
[0047] (2) Place the stirred small beaker or small centrifuge tube containing the mixed solution on a magnet (or magnetic stirrer) to help the magnetic nanoparticles settle, then pour out the supernatant, add deionized water to wash, So repeated 2-3 times.
[0048] (3) Add the cleaned nano-magnetic particles to 5 mL of insulin solution (preparation of insulin solution: 5 mg of insulin is dissolved in 1 mL of 0.01 mol / L hydrochloric acid, then diluted to 5 mL with PBS pH 7.4 - if any precipitation occurs , you can increase ...
Embodiment 2
[0054] Such as Figure 4 As shown, a device for recovering and releasing insulin includes a recovery system I, a magnetic separation system II and a magnetically labeled insulin storage chamber III,
[0055] The recovery system 1 includes: an insulin-rich tube 3 and an electromagnetic coil 12 arranged around the insulin-rich tube 3;
[0056] The magnetic separation system II includes: Y-shaped pipeline, which includes A inlet 5, B outlet 7 and C outlet 8, and a permanent magnet 6 is provided on the side of Y-shaped pipeline B outlet 7;
[0057] The magnetically marked insulin storage chamber III includes: a housing 15, a piston 12 is arranged inside the housing 15, an electromagnetic coil III10 is provided at the left end, and a micromotor 11 is provided at the right end, and the inner space of the housing 15 is divided into a left chamber 37 and a left chamber 37 by the piston 12. The right chamber 38, the piston 12 is provided with a reversing valve 9 communicating with the...
Embodiment 3
[0061] Such as Figure 5 As shown, a control system for an insulin recovery and release device, including:
[0062] A blood glucose sensor for detecting blood glucose concentration;
[0063] The controller is used to receive the blood glucose concentration signal from the blood glucose sensor. When the blood glucose concentration is higher than the set threshold, the electromagnetic coil I and the electromagnetic coil III are de-energized, and the micromotor is started to make the piston reciprocate and release the magnetically labeled insulin; When the blood sugar concentration is lower than the set threshold, the electromagnetic coil I and the electromagnetic coil III are energized, and the micromotor is started to make the piston reciprocate, and the magnetically labeled insulin is recovered; when the blood sugar concentration is normal, the electromagnetic coil I and the electromagnetic coil III are not energized, Turn off the micromotor.
[0064] The working principle o...
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