Lacrimal duct suppository and preparation method thereof
A technology of lacrimal plug and gellan gum, which is applied in the field of medicine, can solve the problems of low structural matching between the lacrimal plug and the lacrimal canaliculus, low mechanical strength, and high mechanical strength, and achieve good curative effect, improve strength, and shear resistance strong effect
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[0031] In some of these embodiments, the preparation method of the polylactic acid-glycolic acid copolymer microspheres (PLGA microspheres) comprises: dissolving PLGA (polylactic acid-glycolic acid copolymer) in dichloromethane to prepare an initial emulsion; The obtained primary emulsion is mixed with the aqueous solution of Tween20 to prepare an emulsion; the obtained emulsion is stirred, filtered and freeze-dried to obtain PLGA microspheres.
[0032] In the present invention, the morphology of PLGA has an important influence on the function of PLGA in the lacrimal plug. When PLGA is added to the lacrimal plug in the form of microspheres, it has a better effect of improving mechanical properties. In addition, the preparation method of PLGA microspheres also has a certain influence on its effect in lacrimal plugs. The present invention selects PLGA to be dissolved in dichloromethane from numerous preparation methods of PLGA microspheres to prepare primary emulsion; The obtain...
Embodiment 1
[0058] The present embodiment provides a kind of lacrimal duct plug, and its preparation method is as follows:
[0059] (1) Prepare 30 mL of an aqueous solution of deacylated gellan gum (1% mass concentration).
[0060] (2) Add 0.9 g of ethyl[3-(dimethylamino)propyl]carbodiimide hydrochloride (EDAC), stir and react for 1 hour, and set the temperature at 20°C.
[0061] (3) Add 0.9 g of L-cysteine hydrochloride, adjust the pH to 5.0 with NaOH, and react for 5 hours.
[0062] (4) The obtained reaction product is intercepted through a dialysis bag (molecular weight cut-off 12kDA-14kDA). The dialysis time was 48 hours, and the concentrated solution was taken as mercaptolated gellan gum.
[0063] (5) Add polylactic-co-glycolic acid (PLGA) microspheres accounting for 0.5 wt% of the mercapto-gellan gum to the prepared mercapto-gellan gum, and stir evenly at 40°C.
[0064] (6) Load the PLGA / mercapto-gellan gum complex into the syringe and store it at low temperature.
Embodiment 2
[0066] The present embodiment provides a kind of lacrimal duct plug, and its preparation method is as follows:
[0067] (1) Prepare 30 mL of an aqueous solution of deacylated gellan gum (2% mass concentration).
[0068] (2) Add 0.5 g of EDAC, stir and react for 1 hour, and set the temperature at 20°C.
[0069] (3) Add 0.5 g of L-cysteine hydrochloride, adjust the pH to 5.0 with NaOH, and react for 5 hours.
[0070] (4) The obtained reaction product is intercepted through a dialysis bag (molecular weight cut-off 12kDA-14kDA). The dialysis time was 48 hours, and the concentrated solution was taken as mercaptolated gellan gum.
[0071] (5) Add PLGA microspheres accounting for 0.5 wt% of mercapto-gellan gum to the prepared mercapto-gellan gum, and stir evenly at 40°C.
[0072] (6) Load the PLGA / mercapto-gellan gum complex into the syringe and store it at low temperature.
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