Laser speckle contrast imaging device and laser speckle contrast imaging method for realizing deep tissue detection

Abstract

The invention discloses a laser speckle contrast imaging device and method for realizing deep tissue detection, and the device comprises a laser light source which is used for irradiating a to-be-detected sample; a beam expander which is used for expanding an irradiation area of laser and making irradiation more uniform; a polarizer which is a linear polarizer; to-be-detected samples which are biological tissues, scattering media, turbid liquid and the like; a semi-transmitting and semi-reflecting mirror which is used for reflecting the light emitted by a laser light source and transmitting the back-scattered light of the to-be-detected samples; a polarization analyzer which is a linear polaroid, the vibration transmission direction of the polarization analyzer being perpendicular to the vibration transmission direction of the polarizer, and the polarization analyzer being used for filtering photons scattered and depolarized from the sample to be detected; an objective lens; a relay lens; a light field camera which is used for collecting and imaging the scattered light to realize depth-of-field expansion of the image. According to the technical scheme, light scattered on the surface and the shallow surface of tissues can be filtered out, the influence of mutual superposition of information of light from different depth levels of the tissues on imaging is eliminated, the contrast ratio degree and the signal-to-noise ratio of a speckle image of the tissue are improved, and deep tissue detection is achieved.

Description

technical field [0001] The invention relates to the field of optical imaging, in particular to a laser speckle contrast imaging device and method for realizing deep tissue detection. Background technique [0002] When coherent light interacts with a random medium, the sensor receives light scattered from different locations in the medium, resulting in a random distribution with destructive or constructive interference, which is laser speckle. The movement of scattering particles in a random medium will cause the phase shift of scattered light, which will cause temporal fluctuations in the speckle pattern. By analyzing this fluctuation, the motion information of the scattering particles can be obtained, and the motion speed of the particles can be characterized by the speckle contrast. This is the working principle of laser speckle contrast imaging technology. [0003] Since the laser speckle contrast imaging technology was proposed in the 1990s, it has been used in cerebra...

AI Technical Summary

Problems solved by technology

When a lens is used to collect scattered light, the light from different depths of the tissue cannot be distinguished, resulting in the mutual influence of information at different depth levels, so it can only measure the shallow surface of the tissue, but cannot accurately detect deeper tissues

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