Yeast recombinant human type I collagen alpha 1 chain protein, synthesis method and application thereof

A collagen and catenin technology, applied in the field of bioengineering, can solve the problems of difficult purification process, complex operation of purification process, difficulty in improving purity, etc., and achieve the effect of improving cutting efficiency, simple fermentation process and short fermentation cycle
CN110964099APending Publication Date: 2020-04-07JIANGSU TRAUTEC MEDICAL TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGSU TRAUTEC MEDICAL TECH CO LTD
Publication Date
2020-04-07

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Abstract

The invention discloses a yeast recombinant human type I collagen alpha 1 chain protein, a synthesis method and application thereof. The recombinant human type I collagen alpha 1 chain protein of theinvention consists of an N-terminal affinity purification marker, a human type I collagen alpha 1 chain mature peptide sequence and a C-terminal affinity purification marker. The bispecific affinity purification markers designed at the two terminals are beneficial to purification of the recombinant protein and are also beneficial to detection and full-length identification of the recombinant protein. The synthesis method comprises the following steps: firstly optimizing collagen gene sequence by utilizing common codons of pichia pastoris at gene level and synthesizing the whole gene artificially, then constructing a recombinant vector through PCR, enzyme digestion, connection and the like, and integrating into yeast artificial chromosome to construct recombinant pichia pastoris engineeringbacteria for secretory expression of the recombinant human type I collagen alpha 1 chain protein. The bispecific affinity purification marker carried by the recombinant human type I collagen alpha 1chain protein of the invention can be subjected to two-step specific purification to easily obtain high-purity products.
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Description

technical field

[0001] The invention relates to the technical field of bioengineering, in particular to a yeast recombinant human type I collagen α1 chain protein, a synthesis method and an application thereof. Background technique

[0002] Collagen is the most abundant protein family in animals, accounting for about 30% of the total protein in animals. Among the 29 collagens found so far, type I collagen has the highest content, accounting for about 90%. In the morphogenesis and cell metabolism of new tissues, it endows new tissues with mechanical strength and biochemical properties; it is mainly distributed in skin, cornea, tendon and other parts, and it plays an important role in maintaining the normal physiological functions and repairing of cells and tissues effect.

[0003] Type I collagen has good biocompatibility, biodegradability, non-cytotoxicity, anti-oxidation and other functions, and can promote cell adhesion and cell proliferation, and can be widely used in m...

Claims

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