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Puerarin pharmaceutical composition with brain injury protection effect

A technology for protection and brain injury, applied in the field of medicine, can solve problems such as research on puerarin compositions that have not been seen

Inactive Publication Date: 2020-04-10
南京温博生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Purple salvia terpene (Przewalskin, CAS number: 119400-87-2) is a natural product isolated from plants, and there is no research on its combination with puerarin

Method used

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  • Puerarin pharmaceutical composition with brain injury protection effect
  • Puerarin pharmaceutical composition with brain injury protection effect
  • Puerarin pharmaceutical composition with brain injury protection effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: Determination of brain protection activity

[0025] 1. Experimental materials

[0026] Healthy male SD rats (body weight 220±20g) were fed in an SPF-grade animal feeding environment, and were adaptively fed for 1 week. The rats were free to eat and drink. Fasting 12h before the operation, free to drink water.

[0027] The purity of puerarin and purple salvia terpene is not less than 98%, and 0.1% DMSO is used in the solvent to aid dissolution.

[0028] 2. Experimental method

[0029] 1. Grouping, modeling and administration

[0030] Healthy male SD rats (body weight 220±20g) were randomly divided into sham operation group, model group, puerarin group, red salvia terpene group, red salvia terpene + puerarin group, 8 rats in each group. Model group, puerarin group, purple salvia terpene group, purple salvia terpene + puerarin group were used to establish cerebral ischemia-reperfusion injury model by suture embolization: the rats were anesthetized and fix...

Embodiment 2

[0039] Embodiment 2: BBB transmittance measurement

[0040] Prepare blood-brain barrier (BBB) ​​in vitro experimental model according to the literature method (Effect of borneol on puerarin passing through simulated blood-brain barrier in vitro, Chinese Herbal Medicine Volume 48, No. 16, August 2017), and determine the passage of drugs through the BBB in vitro model. permeability.

[0041] 1. Experimental materials

[0042] Mouse brain microvascular endothelial cells (bEnd.3) were purchased from Shanghai Yaji Biotechnology Co., Ltd.

[0043] The purity of puerarin and purple salvia terpene is not less than 98%, and 0.1% DMSO is used in the solvent to aid dissolution.

[0044] DMEM high-glucose medium, FBS and penicillin and streptomycin were purchased from Gibco.

[0045] 12-well Transwell cell culture plate (0.4 μm) was purchased from Corning Company.

[0046] 2. Experimental method

[0047] 1. In vitro culture of endothelial cells bEnd.3

[0048] Endothelial cells bEnd...

Embodiment 3

[0062] Embodiment 3: pharmaceutical composition

[0063] A pharmaceutical composition is composed of puerarin B and purple salvia terpene, and the composition can be made into a pharmaceutically acceptable dosage form with pharmaceutically acceptable auxiliary materials for preparing brain-protecting pharmaceutical preparations.

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Abstract

The invention discloses a puerarin pharmaceutical composition with a brain injury protection effect. The research result displays that puerarin has a brain protection effect on rats with cerebral ischemia-reperfusion injury, a cerebral infarction area can be reduced; if the medicine is administrated together with radix salviae miltiorrhizae terpene ether; the brain protection effect of puerarin isfurther enhanced; a blood-brain barrier model proves that the enhancement effect of the radix salviae miltiorrhizae terpene ether on the brain protection efficacy of the puerarin is related to the enhancement of the brain absorption of the puerarin, so that the radix salviae miltiorrhizae terpene ether and the puerarin can be prepared into the pharmaceutical composition to overcome the defect that the blood-brain barrier hinders the absorption of the puerarin in the prior art.

Description

technical field [0001] The invention belongs to the field of medicine and relates to a pharmaceutical composition, in particular to a puerarin pharmaceutical composition with brain damage protection. Background technique [0002] Pueraria lobata is a traditional medicinal and edible plant in my country, with rich chemical components, mainly including isoflavones and their aglycones, triterpenoids, coumarins, etc. Among them, isoflavones and their aglycones are the main active ingredients, mainly puerarin, Mainly daidzin and daidzein. [0003] Puerarin is an isoflavone compound, and its pharmacological activity has been extensively studied since it was isolated and identified in the 1950s. Modern pharmacological studies have found that puerarin has a significant protective effect on animal and cell models such as Alzheimer's disease (AD), Parkinson's disease (PD) and stroke, which has brought good news to the treatment of intractable diseases of the nervous system. [0004] ...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P25/00A61P9/10
CPCA61K31/352A61P9/10A61P25/00A61K2300/00
Inventor 姜东花其他发明人请求不公开姓名
Owner 南京温博生物科技有限公司
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