Reagent for predicting treatment reactivity of children with Kawasaki disease and application of reagent

A technology of detection reagents and kits, which is applied in the fields of genetic engineering and molecular genetics, can solve the problems of comparison and verification of multiple gene analysis, and achieve the effect of low cost and easy operation

Active Publication Date: 2020-04-10
AFFILIATED CHILDRENS HOSPITAL OF CAPITAL INST OF PEDIATRICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these studies are studies on a certain gene or a certain gene pathway in different populations,

Method used

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  • Reagent for predicting treatment reactivity of children with Kawasaki disease and application of reagent
  • Reagent for predicting treatment reactivity of children with Kawasaki disease and application of reagent
  • Reagent for predicting treatment reactivity of children with Kawasaki disease and application of reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: Detection of susceptibility genes of Kawasaki disease IVIG unresponsive type

[0051] We selected 231 KD children (77 IVIG non-responsive KD children and 154 IVIG sensitive KD children) who were hospitalized in the Department of Cardiology, Children's Hospital Affiliated to Capital Institute of Pediatrics from December 2015 to August 2019. The selected KD diagnostic criteria include: fever for more than 5 days, accompanied by 4 of the following 5 clinical manifestations, and after excluding other diseases, it is diagnosed as Kawasaki disease: (1) Changes in limbs: rigid edema of hands and feet in the acute stage, fingers (toes) in the recovery stage ) terminal membranous desquamation; (2) erythema multiforme; (3) non-suppurative hyperemia of conjunctiva; (4) oral mucosal hyperemia, red bayberry tongue; (5) cervical lymph node enlargement; Less than 4 items, but coronary artery damage in echocardiography can be diagnosed as incomplete Kawasaki disease. The di...

Embodiment 2

[0106] Example 2: Establishment of predictive scoring model for IVIG resistance in children with KD - nomogram creation and verification The old predictive model established by our hospital based on laboratory biochemical indicators is shown in Table 7.

[0107] Table 7 Prediction models based on biochemical indicators

[0108]

[0109] Construction of a new predictive model for gene joint laboratory indicators:

[0110] In this study, the 4 SNPs related to the IVIG-responsive type of KD children and the prediction model indicators based on the previous laboratory biochemical indicators in our hospital were used as variables, and 6 patients with incomplete laboratory indicators were excluded from the 231 KD patients. Finally, 225 KD children (75 IVIG non-responsive KD children and 150 IVIG sensitive KD children) were included in the next step to build a new prediction model. The nomogram for predicting IVIG response made by R software assigns scores to each variable as fol...

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Abstract

The invention provides a reagent for predicting treatment reactivity of children with Kawasaki disease and an application of the reagent, and mainly provides the reagent for detecting an intravenous human immunoglobulin (IVIG) non-responsive Kawasaki disease susceptibility gene and a kit containing the reagent. The reagent can evaluate the reactivity of the children with the Kawasaki disease to IVIG treatment by detecting 5 SNP loci of 3 genes (rs10056474 or rs746994 of an SMAD5 gene, rs76863441 of a PLA2G7 gene, and/or rs16944 or rs1143627 of an IL-1B gene) of a patient. The kit has the characteristics of simple operation and low costs; and the reagent and kit provided by the invention can provide a basis for screening IVIG non-responsive high-risk children as early as possible and guiding clinical doctors to take reasonable intervention and treatment measures, and is in line with the development trend of precision medicine.

Description

[0001] field of invention [0002] The invention belongs to the field of genetic engineering and molecular genetics, in particular, relates to a reagent for predicting Kawasaki disease treatment responsiveness and its application, especially for detecting no response to intravenous injection of human immunoglobulin (intravenousimmunoglobulin, IVIG) Reagents and applications of Kawasaki disease susceptibility genes. [0003] technical background [0004] Kawasaki disease (KD), also known as mucocutaneous lymph node syndrome (MCLS), is an acute febrile and eruptive disease that mainly occurs in children under 5 years old, with systemic vascular inflammation as the main lesion. Kawasaki disease is currently the most common cause of acquired heart disease in children. The main complication of Kawasaki disease is coronary artery injury, including coronary artery dilation, coronary aneurysm, coronary thrombosis, stenosis, occlusion and even sudden death. Seriously affect the qualit...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/106C12Q2600/156C12Q2600/172
Inventor 李晓惠姜茜孟莉姚玮杨帅张明明林瑶刘杨李爱杰石琳
Owner AFFILIATED CHILDRENS HOSPITAL OF CAPITAL INST OF PEDIATRICS
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