Full-automatic fluorescence labeling single-cell suspension preparation device and matched sample loading tube

Abstract

The invention discloses a full-automatic fluorescence labeling single-cell suspension preparation device and a matched sample loading tube. The device is composed of a sample loading tube frame rotating device, a sample loading tube frame, a sample loading tube frame top cover, a sample adding device, a comprehensive controller and the like. The matched sample loading tube is composed of a sampletube body, an incubation cup, an injector rod, an injector rod push plate, an injector rod sealing ring, an injector rod cavity, a filter membrane supporting frame, a filter membrane supporting framesealing ring, a filter membrane and the like. The device and the matched sample loading tube can automatically treat human or animal peripheral blood specimens, and comprise the steps of sample adding, dyeing, hemolysis, white blood cell separation, white blood cell suspension and the like, so that the labor intensity and personal errors are reduced. The matched sample loading tube adopts a filtermembrane filtration principle, and erythrocyte fragments and waste liquid are removed in batches by using an injector instead of a vacuum pump, so that fluorescently-labeled red blood cells can be separated in an extremely short time, the size of corresponding equipment is reduced, the cost of the vacuum pump is reduced, and the space and the cost are saved.

Description

technical field [0001] The invention belongs to the field of biomedical equipment, and in particular relates to a PLC-controlled automatic fluorescence-labeled single-cell suspension preparation device and a matching sample loading tube, which are used for automatically processing peripheral blood samples of humans or animals. Background technique [0002] At present, for the detection of peripheral blood leukocyte surface antigens in humans or animals, the collected peripheral blood samples must be processed before the detection. The existing treatment methods are roughly divided into five steps: drawing blood samples and adding them to experimental vessels, adding fluorescent-labeled antibody staining, adding hemolysin (or hemolyzing agent) for hemolysis, separating labeled white blood cells and suspending labeled white blood cells in liquid, and the experimental process of treatment It has the characteristics of precious experimental consumables, cumbersome experimental p...

AI Technical Summary

Problems solved by technology

Although the centrifugation method is economical and convenient, it needs to be equipped with a centrifuge, and it will cause damage and loss of white blood cells; the density gradient separation method and the magnetic bead adsorption method require more expensive separation liquid and adsorption magnetic beads, which are time-consuming and cannot avoid the loss of white blood cells ;Membrane filtration requires additional filter vessel and vacuum pump

Centrifuges are common equipment in the laboratory, so centrifugation is the most common way to separate white blood cells. However, this method requires manual transfer of the experimental vessels to the centrifuge for centrifugation, and after centrifugation, it is still necessary to manually sort the experimental vessels in order to continue adding samples. Machine detection is labor-intensive and error-prone

[0004] At present, some sample processing devices that replace manual work have been launched on the market. For example, after staining and hemolysis, the step of separating white blood cells is omitted, and the machine is directly tested, which is called no-wash mode. It has adverse effects on the subsequent detection results, and it is easy to cause faults such as blockage of the detection device, so the effect of popularization and application is not good

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