Single cell separation and collection apparatus

A collection device and separation device technology, which is applied in the field of medical devices, can solve the problems of incomplete removal of impurity cells, expensive instruments, and unstable manual absorption of single cells, and achieve the effects of simple structure, stable absorption process, and low cost

Inactive Publication Date: 2020-04-14
常州市武进人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problems that the impurity cells in the high-throughput sorting instrument in the prior art cannot be completely removed, the instrument itself is expensive, and the manual suction of single cells is unstable. and collection device

Method used

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  • Single cell separation and collection apparatus

Examples

Experimental program
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Effect test

Embodiment 1

[0031] like figure 2 , the support 3 is fixed on the side of the microscope 1, the support 3 includes a column 31 and a beam 32 installed on the column, a height adjustment device is provided between the column 31 and the beam 32, and the installation pipe 6 is rotatably connected to the end of the beam 32. The mounting tube 6 can be rotated to a position away from the stage of the microscope 1 , so that work such as placing the petri dish 2 or cleaning the stage of the microscope 1 can be performed without colliding with the suction pipe assembly 7 .

[0032] The suction pipeline is provided with a first branch 12 and a second branch 13, the first branch 12 is close to the inlet of the suction pipeline, and the second branch 13 is close to the outlet of the suction pipeline, and the suction device includes a The blowing pump 14 and the suction pump 15 arranged on the second branch 13, the first branch 12 and the second branch 13 are provided with a shut-off valve 6, and the ...

Embodiment 2

[0037] like Figure 3-4 , the bracket 3 is fixed on the frame of the microscope, the installation tube 6 is rotatably connected to the bracket 3, and the installation tube 6 rotates in a direction close to the stage and away from the stage.

[0038] The suction device comprises a fluid pump 4 arranged in the liquid suction pipeline. In the liquid suction pipeline, a purification branch 11 is provided upstream of the fluid pump 4. The purification branch 11 is provided with a sterile air filter cotton 5. The purification branch 11 is also provided with There is a shut-off valve 6.

[0039] The bracket 3 is rotated to connect the jaws 9, the installation pipe 6 is clamped in the jaws 9, and the bracket 3 also has a fixed structure for fixing the rotational position of the jaws 9. The fixing structure is a fixing pin or a buckle.

[0040] Rotate and connect the claw 9 on the bracket 3, and the installation tube 6 is clamped in the claw 9. When installing, just open the screw on...

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Abstract

The invention relates to the technical field of medical instruments, and particularly relates to a single cell separation and collection apparatus. The apparatus comprises a microscope, a culture dishis placed on the objective table of the microscope, a support is fixed on the rack of the microscope, a suction pipe assembly is installed on support and comprises a suction pipe, the suction pipe extends into the culture dish and sucks a single cell, the tail end of the suction pipe assembly is communicated with a liquid suction pipeline, and a suction device is arranged in the liquid suction pipeline. The single cell separation and collection apparatus replaces manual work to suck single cells, has a stable sucking process and has a high efficiency.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a device for separating and collecting single cells. Background technique [0002] In natural science and basic medicine, we need to select our interested or specific immune cells or adherent tumor cells from the primary cultured cells or solid tumor cells, or after transfection and drug addition screening, the interested Specific cells are isolated and cultured or tested. At present, imported equipment commonly used in laboratories for cell separation (such as flow sorters, microporous chip separators, etc.), related equipment is not only expensive, but also can only separate a certain percentage of specific cells, which contain miscellaneous cells It cannot be eliminated; while the mouth pipette technology using manual separation requires a high degree of proficiency of the operator, and the micromanipulation technology is currently aimed at yeast cells. Therefore, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/22C12M1/12C12M1/00
CPCC12M23/10C12M23/48C12M33/04C12M47/04
Inventor 刘迁金建华陆文斌杨春霞李文晶沈颖
Owner 常州市武进人民医院
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