Fusarium diacetate competitive barometric pressure immunosensor and detection method
A fusarium-enol-competitive, fusarium-enol technology, which can be used in instruments, measuring devices, scientific instruments, etc., can solve the problems of increasing the risk of increasing the quality and safety of agricultural products, complicated sample processing, and mycotoxin contamination, etc. The effect of strong catalytic ability, strong signal amplification ability and low price
Active Publication Date: 2021-10-08
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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Problems solved by technology
The production of agricultural products involves many links, and mycotoxin pollution is inevitable. my country has set limits on the main mycotoxins in food, but there is no limit on snake toxins
Thereby increasing the hidden dangers of the quality and safety of agricultural products
[0003] The existing detection method of Fusarium diacetate is mainly based on the laboratory confirmatory detection technology of large-scale instruments such as high-performance liquid chromatography, the sample processing is complicated, and it is not convenient for on-site detection.
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Embodiment 1
[0056] Example 1 Preparation process of gold core platinum shell particle and gold core platinum shell particle modified goat anti-mouse antibody
[0057] Gold-core platinum-shell particles were synthesized as follows: 10 mL of AuNPs solution was added to 200 μL of 3.86 mM H2PtCL4. After heating the mixture to 80°C for 5 minutes with magnetic stirring, 400 μL, 10 mM ascorbic acid was added dropwise to the mixture and maintained at 80°C for 30 minutes. The obtained gold-core platinum-shell particle aqueous solution is naturally cooled and stored for future use.
[0058] Preparation of gold-core-platinum-shell particle-modified antibody: Add 4ug of anti-mouse secondary antibody (goat anti-mouse antibody) to the gold-core platinum-shell particle and mix well. After incubation for 1-3 hours, add blocking agent containing Tween-20 and inert protein Blocked in the solution for 1-3h to obtain a gold core platinum shell particle solution of the modified goat anti-mouse antibody. Cen...
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Abstract
The invention relates to a diacetate fusarium diacetate competitive air pressure immunosensor and a detection method. Fusarenol diacetate competitive barometric pressure immunosensor, including anti-falsarenol diacetate monoclonal antibody stored separately, enzyme labeling strips, and Fusarenol diacetate-coated enzyme labeling strips Semiglutamic acid-whey protein conjugate, hydrogen peroxide solution, platinum nanoparticles or gold-core platinum-shell nanoparticles of surface-modified sheep anti-mouse antibody and an air pressure detection sensor for detecting air pressure; The Fusarium enol monoclonal antibody is produced by the hybridoma cell strain DAS5G11E7 with the preservation number CCTCC NO: C201881. This method has the advantages of high sensitivity, portability, low cost and easy operation.
Description
technical field [0001] The invention relates to an air pressure immune sensor of a diacetate diacetate competition type air pressure immunosensor and a method for detecting the diacetate diacetate content using the same. Background technique [0002] Diacetoxyscirpenol (Diacetoxyscirpenol, DAS), also known as snake-like toxin, belongs to a kind of trichothecene mycotoxins, mainly produced by Fusarium pirisporium, Fusarium equiseti and Fusarium spp. Born by the metabolism of spores. It mainly pollutes grain and feed, and its toxicity is high, which can damage human and animal bone marrow and other hematopoietic organs, brain and central nervous cell degeneration, lymph nodes, testis and thymus, etc. Therefore, snake toxin pollution is a major risk and hidden danger that threatens the quality and safety of agricultural products and the development of agricultural industries. The production of agricultural products involves many links, and mycotoxin pollution is inevitable. m...
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IPC IPC(8): G01N33/543G01N33/577
CPCG01N33/54346G01N33/577G01N2333/37
Inventor 张兆威唐晓倩王督张奇李培武
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI