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Carbon dots with tumor targeting function as well as preparation method and application thereof

A technology of tumor targeting and carbon dots, which is applied in the direction of non-active ingredient medical preparations, medical preparations containing active ingredients, anti-tumor drugs, etc. and organ toxicity and side effects, to achieve the effect of improving lethality, cheap and easy-to-obtain raw materials, and realizing targeted delivery

Inactive Publication Date: 2020-04-17
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Doxorubicin is a kind of spectrum tumor antibiotic, which can effectively inhibit the synthesis of RNA and DNA, thereby killing tumor cells. At the same time, it caused serious toxic side effects to normal cells and organs.

Method used

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  • Carbon dots with tumor targeting function as well as preparation method and application thereof
  • Carbon dots with tumor targeting function as well as preparation method and application thereof
  • Carbon dots with tumor targeting function as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for preparing carbon dots with tumor targeting function, specifically: weighing 10 mg of folic acid in 10 ml of ultrapure water, and ultrasonicating for 15 minutes to obtain a uniformly dispersed folic acid solution. Then transfer the folic acid solution to a 100 ml hydrothermal reaction kettle and conduct a hydrothermal reaction at 200°C for 8 hours. After the reaction is completed, cool down to room temperature naturally, and remove large particles of insoluble matter by high-speed centrifugation. The filtrate is dialyzed through a 500-1000Da dialysis bag. If there are small particles, dialyze for 24 hours and change the water every 4 hours. After dialysis, freeze-dry with a freeze dryer to obtain carbon dot CDs.

[0027] A nanocomposite containing the above-mentioned carbon dots with tumor targeting function, which dissolves the carbon dots in ultrapure water to obtain a 3mg / ml carbon dot solution, then adds doxorubicin with a mass of 0.05% of the carbon dot...

Embodiment 2

[0029] A method for preparing carbon dots with a tumor targeting function, specifically: weighing 10 mg of folic acid in 15 ml of ultrapure water, and ultrasonicating for 15 minutes to obtain a uniformly dispersed folic acid solution. Then transfer the folic acid solution to a 100 ml hydrothermal reaction kettle and conduct a hydrothermal reaction at 180°C for 12 hours. After the reaction, cool down to room temperature naturally, high-speed centrifugation to remove large particles, and the filtrate is dialyzed through a 500-1000Da dialysis bag. If there are small particles, dialyze for 24 hours and change the water every 4 hours. After dialysis, freeze-dry with a freeze dryer to obtain carbon dot CDs.

[0030] A nanocomposite containing the above-mentioned carbon dots with tumor targeting function, which dissolves the carbon dots in ultrapure water to obtain a 4 mg / ml carbon dot solution, then adds doxorubicin with a mass of 0.08% of the carbon dots, and avoids light and ultra...

Embodiment 3

[0032] A method for preparing carbon dots with tumor-targeting function, specifically: weighing 10 mg of folic acid in 20 ml of ultrapure water, and ultrasonicating for 15 minutes to obtain a uniformly dispersed folic acid solution. Then transfer the folic acid solution to a 100 ml hydrothermal reaction kettle and conduct a hydrothermal reaction at 240°C for 6 hours. After the reaction is completed, cool it down to room temperature naturally, and remove the large particles by high-speed centrifugation. The filtrate is dialyzed through a 500-1000Da dialysis bag. If there are small particles, dialyze for 24 hours and change the water every 4 hours. After dialysis, freeze-dry with a freeze dryer to obtain carbon dot CDs.

[0033] A nanocomposite containing the above-mentioned carbon dots with tumor targeting function, which dissolves the carbon dots in ultrapure water to obtain a 2 mg / ml carbon dot solution, then adds doxorubicin with a mass of 0.10% of the carbon dots, and avoid...

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Abstract

The invention relates to a preparation method of carbon dots with a tumor targeting function, which comprises the following steps: uniformly dispersing folic acid into ultrapure water to obtain a folic acid solution, carrying out hydrothermal reaction at 180-240 DEG C for 6-12 hours, cooling to room temperature after the reaction is finished, carrying out solid-liquid separation, dialyzing an obtained filtrate, and carrying out freeze drying to obtain the carbon dots. According to the invention, folic acid is used as a raw material to synthesize the low-toxicity and environment-friendly fluorescent carbon dots, and the carbon dots have a remarkable tumor targeting function. According to the invention, the chemotherapeutic drug adriamycin is used as a model drug and is loaded on the surfaces of the carbon dots to realize targeted delivery of the chemotherapeutic drug, so that adriamycin is enriched in tumor cells, and the lethality of the adriamycin to tumor tissues is improved.

Description

technical field [0001] The invention belongs to the technical field of carbon dot preparation, and in particular relates to a carbon dot with tumor targeting function, a preparation method and an application. Background technique [0002] At present, nano-carrier systems for heavy metals, semiconductors, and small organic molecules have disadvantages such as expensive raw materials, high toxicity, and complex synthesis processes. In order to overcome the above-mentioned shortcomings in the prior art, it is urgent to design a simple, low-cost, and low-toxicity delivery system. Carbon quantum dots have stable optical properties, small size (less than 10 nm), high stability, low toxicity, good water solubility, easy synthesis, and surface functional modification. It has a good application prospect. Doxorubicin is a kind of spectrum tumor antibiotic, which can effectively inhibit the synthesis of RNA and DNA, thereby killing tumor cells. At the same time, it has caused seriou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/04A61K47/52A61K31/704A61P35/00C01B32/15
CPCA61K31/704A61K47/02A61K47/52A61P35/00C01B32/15
Inventor 杨晓静王瑛盈赵美霞
Owner HENAN UNIVERSITY
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